Dental Enamel Irradiated with Infrared Diode Laser and Photo-Absorbing Cream: Part 2-EDX Study

Autor(es)

de Sant'Anna, Giselle Rodrigues ^{[1, 2, 3]} ; Pereira dos Santos, Edson Aparecido ^[1] ; Silva Soares, Luis Eduardo ^{[1, 4]} ; do Espirito Santo, Ana Maria ^[1] ; Martin, Airton Abrahao ^[1] ; Duarte, Danilo Antonio ^[2] ; Pacheco-Soares, Cristina ^[5] ; Brugnera, Jr., Aldo ^[6]

Afiliação do(s) autor(es)

^[1] Vale do Paraiba Univ, UNIVAP, Inst Res & Dev, IP&D, Lab Biomed Vibrat Spect, LEVB, Sao Paulo - Brazil

^[2] Cruzeiro do Sul Univ, UNICSUL, Sao Paulo - Brazil

^[3] Dent Laser Ctr, Sao Paulo - Brazil

^[4] Fac Hlth Sci FCS, Dent Mat Dept, Sao Paulo - Brazil

^[5] Vale do Paraiba Univ, UNIVAP, Inst Res & Dev, IP&D, Cell & Tissue Lab, Sao Paulo - Brazil

^[6] Vale do Paraiba Univ, UNIVAP, Inst Res & Dev, IP&D, Dent Laser Ctr, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photomedicine and Laser Surgery; v. 27, n. 5, p. 771-782, OCT 2009.
• Citações Web of Science: 10

Resumo

Objective: The effects of laser-induced compositional changes on the enamel were investigated by energy-dispersive X-ray fluorescence spectrometry (mu-EDX). After cariogenic challenge, we administered treatment of low-level infrared diode laser and a photo-absorbing cream (used to intensify the superficial light absorption). Background Data: Dental caries is considered the most prevalent oral disease. A simple and noninvasive caries preventive regimen is treating tooth enamel with a laser, either alone or in combination with fluoride, which reduces enamel solubility and dissolution rates. High power lasers are still not widely used in private practice. Low-power near-infrared lasers may be an alternative approach. Energy-dispersive mu-EDX is a versatile and nondestructive spectroscopic technique that allows for a qualitative and quantitative elemental analysis of inorganic enamel components, such as calcium and phosphorus. Materials and Methods: Twenty-four extracted or exfoliated caries-free deciduous molars were divided into six groups: 1) control group (CTR-no treatment); 2) infrared laser treatment (L) (lambda = 810 nm, 100 mW/cm(2), 90 sec, 4.47 J/cm(2), 9 J); 3) infrared laser irradiation and photo-absorbing agent (CL); 4) photo-absorbing agent alone (C); 5) infrared laser irradiation and fluoridated photo-absorbing agent (FCL); and 6) fluoridated photo-absorbing agent alone (FC). Samples were analyzed using mu-EDX after two sets of treatments and pH cycling cariogenic challenges. Results: The CL group showed statistically significant increases in calcium and phosphorus (wt%) compared with the CTR group. The Ca/P ratio was similar in the FCL and CTR groups. There was a significant laser-induced reduction compared with the CTR group, and there was a possible modification of the organic balance content in enamel treated with laser and cream. Conclusion: mu-EDX may be able to detect compositional changes in mineral phases of lased enamel under cariogenic challenge. Our results suggest that with a combined laser and photo-absorbing agent (CL) treatment, there was a possible disorganization of organic content in the tooth enamel with hydroxyapatite crystal reordering and reorganization. (AU)

• Processo FAPESP: 01/14384-8 - Estudo de células neoplásicas por espectroscopia de infravermelho próximo
• Beneficiário: Airton Abrahao Martin
• Modalidade de apoio: Auxílio à Pesquisa - Regular