An efficient and rapid DNA minipreparation procedure suitable for PCR/SSR and RAPD analyses in tropical forest tree species

Autor(es)

Alzate-Marin, Ana Lilia ^[1] ; Guidugli, Marcela Corbo ^{[2, 1, 3]} ; Soriani, Hilda Hildebrand ^{[2, 1, 3]} ; Martinez, Carlos Alberto ^[3] ; Mestriner, Moacyr Antonio ^[1]

Afiliação do(s) autor(es)

^[1] Fac Med Ribeirao Preto, Dept Genet, Lab Genet Vegetal, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Archives of Biology and Technology; v. 52, n. 5, p. 1217-1224, SEP-OCT 2009.
• Citações Web of Science: 19

Resumo

An efficient and rapid DNA minipreparation modified method for frozen samples was developed for five tropical tree species: Copaifera langsdorffii, Hymenaea courbaril, Eugenia uniflora, Tabebuia roseo alba and Cariniana estrellensis. This procedure that dispenses the use of liquid nitrogen, phenol and the addition of proteinase K, is an adaptation of the CTAB-based DNA extraction method. The modifications included the use of PVP to eliminate the polyphenols, only one chloroform-isoamyl alcohol step and the addition of RNase immediately after extraction with chloroform. The yields of the DNA samples ranged from 25.7 to 42.1 µg from 100 mg leaf tissue. The DNA samples extracted by this method were successfully used for PCR (SSR and RAPD) analyses in these five and other twelve tropical tree species. (AU)

• Processo FAPESP: 03/04199-4 - Uso de marcadores SSR na análise genética de árvores matrizes vs. populações derivadas que formam parte do Banco de Germoplasma da Floresta da USP-RP
• Beneficiário: Ana Lilia Alzate Marin
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores