In vitro analysis of inhibitory effects of the antibacterial monomer MDPB-containing restorations on the progression of secondary root caries

Autor(es)

Thome, Thais ^[1] ; Mayer, Marcia P. A. ^[2] ; Imazato, Satoshi ^[3] ; Geraido-Martins, Vinicius R. ^[1] ; Marques, Marcia M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Odontol, Dept Dent, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508900 Sao Paulo - Brazil

^[3] Osaka Univ, Grad Sch Dent, Dept Restorat Dent & Endodontol, Osaka - Japan

• Tipo de documento: Artigo Científico
• Fonte: Journal of Dentistry; v. 37, n. 9, p. 705-711, SEP 2009.
• Citações Web of Science: 37

Resumo

Objective: This study aimed to analyze in vitro inhibitory effects of restorative materials containing the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) on the formation of artificial secondary root caries lesions. Methods: Class V cavities (2 mm x 2 mm) were prepared in 75 human root fragments. Specimens were randomly divided into five groups (n = 15 fragments per group) and restored as follows: (I) MDPB-free adhesive system + MDPB-free composite (negative control); (II) resin modified glass ionomer (RM-GIC; positive control); (III) MDPB-free adhesive system + MDPB-containing composite (2.83% MDPB); (IV) MDPB-containing adhesive system + MDPB-free composite; M MDPB-containing adhesive system + MDPB-containing composite. Artificial secondary root caries lesions were produced by a biological artificial caries challenge. The restored specimens were immersed into a culture medium containing Streptococcus mutans and sucrose for 15 days. Histological slices (80 +/- 20 mu m) of the specimens were used for measuring the mean depths of the artificial lesions produced in both margins of the restorations using polarized light microscopy. Results were expressed in percentage related to the mean depth of the negative control, considered 100%. Data were compared by ANOVA followed by the Tukey's test (p <= 0.05). Results: The depths of lesions adjacent to cavities filled with RM-GIC (GII; 85.17 +/- 15.2%) were significantly (p < 0.01) shallower than those adjacent to restorations with MDPB-free composite (GI; 100.00 +/- 10.04%), despite the presence of MDPB in the adhesive system (GIV; 101.95 +/- 21.32%). The depths of lesions adjacent to cavities restored with MDPB-containing composite (GIII; 82.68 +/- 12.81% and GV; 85.65 +/- 15.42%), despite the adhesive system used, were similar to those of RM-GIC (GII). Mean lesions depths in these groups decreased from 13% (GV) to 17% (GIII) in relation to the negative control (GI). Conclusions: MDPB-containing composite inhibits the progression of artificial secondary root caries lesions regardless of adhesive systems. (C) 2009 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 04/10377-5 - Analise in vitro do efeito inibitorio de restauracoes contendo o monomero antibacteriano mdpb na formacao de caries secundarias em raizes dentarias.
• Beneficiário: Marcia Martins Marques
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/56158-5 - Analise in vitro do efeito inibitorio de restauracoes contendo o monomero antibacteriano mdpb na formacao das caries secundarias em raizes dentarias.
• Beneficiário: Thais Thome Feldens
• Modalidade de apoio: Bolsas no Brasil - Doutorado