Genetic polymorphism in brazilian microcystis spp. (Cyanobacteria) toxic and non-toxic through RFLP-PCR of the cpcBA-IGS

Autor(es)

Bittencourt-Oliveira, Maria do Carmo ^[1] ; Costa Cunha, Maristela Case ^[2] ; Moura, Ariadne do Nascimento ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Ciencias Biol, Escola Super Agr Luiz Queiroz, BR-13418900 Piracicaba, SP - Brazil

^[2] Univ Fed Rural Pernambuco, Dept Biol, Recife, PE - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Archives of Biology and Technology; v. 52, n. 4, p. 901-909, JUL-AUG 2009.
• Citações Web of Science: 9

Resumo

The escalating occurrence of cyanobacterial toxic blooms demands a better understanding of genetic variability as an auxiliary expedient in species identification, collaborating with the monitoring of water destined to public supply. This study aimed at the unraveling of genetic polymorphism in the toxic and nontoxic strains of Microcystis (Cyanobacteria) species, isolated from diverse Brazilian localities through the RFLP-PCR technique applied to the c-phycocyanin encoding operon and its intergenic spacer (cpcBA-IGS). Eighteen strains belonging to M. aeruginosa, M. panniformis, M. protocystis and M. wesenbergii, plus two other unidentified strains, were analyzed by means of the morphological and molecular data. The molecular data constituted three groups with low similarity values unrelated to the geographical origin, toxicity or morphospecies. A high genetic variability among the studied populations was unveiled by the results. Brazilian populations of Microcystis spp. displayed high genetic diversity when compared to those from Australia, Japan, United States and Europe. This ample genetic diversity could be observed through the diverse eletrophoretic profiles obtained among the strains from a single species. The presence of toxic and non-toxic strains was observed in the same species, as M. aeruginosa. (AU)

• Processo FAPESP: 00/05157-5 - Identificação de populações de cianobactérias (Cyanobacteria/Cyanophyta) hepatotóxicas através do gene que codifica para a microcistina sintetase
• Beneficiário: Maria Do Carmo Bittencourt de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 03/05773-6 - Filogenia molecular de Microcystis (Cyanobacteria) tóxicas e não-tóxicas de ecossistemas aquáticos tropicais
• Beneficiário: Maria Do Carmo Bittencourt de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular