Detection of metallo-beta-lactamases-encoding genes in environmental isolates of Aeromonas hydrophila and Aeromonas jandaei

Autor(es)

Balsalobre, L. C. ^[1] ; Dropa, M. ^[1] ; Lincopan, N. ^{[2, 3]} ; Mamizuka, E. M. ^[2] ; Matte, G. R. ^[1] ; Matte, M. H. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Publ Hlth, Publ Hlth Lab, BR-01246904 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Pharm, Dept Clin Anal, BR-01246904 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-01246904 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Letters in Applied Microbiology; v. 49, n. 1, p. 142-145, JUL 2009.
• Citações Web of Science: 13

Resumo

Aims: To determine the prevalence and expression of metallo-beta-lactamases (MBL)-encoding genes in Aeromonas species recovered from natural water reservoirs in southeastern Brazil. Methods and Results: Eighty-seven Aeromonas isolates belonging to Aeromonas hydrophila (n = 41) and Aer. jandaei (n = 46) species were tested for MBL production by the combined disk test using imipenem and meropenem disks as substrates and EDTA or thioglycolic acid as inhibitors. The presence of MBL genes was investigated by PCR and sequencing using new consensus primer pairs designed in this study. The cphA gene was found in 97.6% and 100% of Aer. hydrophila and Aer. jandaei isolates, respectively, whereas the acquired MBL genes bla(IMP), bla(VIM) and bla(SPM-1) were not detected. On the other hand, production of MBL activity was detectable in 87.8% and 10.9% of the cphA-positive Aer. hydrophila and Aer. jandaei isolates respectively. Conclusions: Our results indicate that cphA seems to be intrinsic in the environmental isolates of Aer. hydrophila and Aer. jandaei in southeastern Brazil, although, based on the combined disk test, not all of them are apparently able to express the enzymatic activity. Significance and Impact of the Study: These data confirm the presence of MBL-producing Aeromonas species in natural water reservoirs. Risk of water-borne diseases owing to domestic and industrial uses of freshwater should be re-examined from the increase of bacterial resistance point of view. (AU)

• Processo FAPESP: 07/02238-3 - Genotipagem de Aeromonas spp. isoladas de amostras ambientais, de fatores de virulência e genes de resistência
• Beneficiário: Maria Helena Matte
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/02266-7 - Pesquisa de genes de virulência e de resistência em Aeromonas jandaei e Aeromonas hydrophila provenientes de amostras ambientais
• Beneficiário: Livia Carminato Balsalobre
• Modalidade de apoio: Bolsas no Brasil - Mestrado