B-1 cells modulate oral tolerance in mice

Autor(es)

De-Gennaro, Luiz Antonio ^[1] ; Popi, Ana Flavia ^[1] ; de Almeida, Sandro Rogrio ^[2] ; Lopes, Jose Daniel ^[1] ; Mariano, Mario ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Discipline Immunol, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Chem Toxicol, Sch Pharmaceut Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Immunology Letters; v. 124, n. 2, p. 63-69, JUN 4 2009.
• Citações Web of Science: 8

Resumo

Although the origin and functions of B-1 cells are controversial, they are considered as a cellular element of innate immunity due to their ability to produce natural autoantibodies of the IgM type. These antibodies are encoded by a relatively limited repertoire of V genes, and their resulting diversity is smaller than that produced by conventional B cells. B-1 cells constitute the larger fraction of B cells in the peritoneal cavity and migrate to non-specific inflammation sites. In addition, they contribute to the production of IgA antibodies in the intestinal lamina propria. It has been demonstrated that they participate in the induction and maintenance of peripheral tolerance. Herein, the participation of B-1 cells in inducing oral tolerance is evaluated. Unexpectedly, BALB/Xid mice, the animals deficient in B-1 cells, are not tolerized to OVA but instead are responsive to oral immunization. Conversely, BALB/c mice respond to oral tolerance to this antigen. We used these biological characteristics of these animals to investigate whether BA cells are involved in the induction of oral tolerance to OVA. Results show that B-1 cells from BALB/c mice, treated orally with OVA and adoptively transferred to BALB/Xid mice were able to suppress local hypersensitivity reaction and lymphoproliferative cellular response observed in BALB/.Xid mice. These data demonstrate that B-1 cells have regulatory properties and are involved in the induction of oral tolerance. (C) 2009 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 04/08506-1 - Células B-1: origem, diferenciação e participação na inflamação, cicatrização e rejeição de enxerto
• Beneficiário: Mario Mariano
• Modalidade de apoio: Auxílio à Pesquisa - Temático