Adipose Overexpression of Desnutrin Promotes Fatty Acid Use and Attenuates Diet-Induced Obesity

Autor(es):Mostrar menos -

Ahmadian, Maryam ^[1] ; Duncan, Robin E. ^[1] ; Varady, Krista A. ^[1] ; Frasson, Danubia ^[1] ; Hellerstein, Marc K. ^[1] ; Birkenfeld, Andreas L. ^[2] ; Samuel, Varman T. ^[2] ; Shulman, Gerald I. ^[2] ; Wang, Yuhui ^[1] ; Kang, Chulho ^[3] ; Sul, Hei Sook ^[1]

Afiliação do(s) autor(es)

^[1] Univ Calif Berkeley, Dept Nutr Sci & Technol, Berkeley, CA 94720 - USA

^[2] Yale Univ, Sch Med, Dept Internal Med, New Haven, CT 06510 - USA

^[3] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 - USA

• Tipo de documento: Artigo Científico
• Fonte: Diabetes; v. 58, n. 4, p. 855-866, APR 2009.
• Citações Web of Science: 104

Resumo

OBJECTIVE-To investigate the role of desnutrin in adipose tissue triacylglycerol (TAG) and fatty acid metabolism. RESEARCH DESIGN AND METHODS-We generated transgenic mice overexpressing desnutrin (also called adipose triglyceride lipase {[}ATGL]) in adipocytes (aP2-desnutrin) and also performed adenoviral-mediated overexpression of desnutrin in 3T3-L1CAR Delta 1 adipocytes. RESULTS-aP2-desnutrin mice were leaner with decreased adipose tissue TAG content and smaller adipocyte size. Overexpression of desnutrin increased lipolysis but did not result in increased serum nonesterified fatty acid levels or ectopic TAG storage. We found increased cycling between diacylglycerol (DAG) and TAG and increased fatty acid oxidation in adipocytes from these mice, as well as improved insulin sensitivity. CONCLUSIONS-We show that by increasing lipolysis, desnutrin overexpression causes reduced adipocyte TAG content and attenuation of diet-induced obesity. Desnutrin-mediated lipolysis promotes fatty acid oxidation and re-esterification within adipocytes. Diabetes 58:855-866, 2009 (AU)

• Processo FAPESP: 05/54620-3 - Geração de glicerol-3-fosfato pelos tecidos adiposos epididimal e retroperitoneal: efeito da desnervação
• Beneficiário: Danúbia Frasson
• Modalidade de apoio: Bolsas no Brasil - Doutorado