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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Glu298Asp eNOS gene polymorphism causes attenuation in nonexercising muscle vasodilatation

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Autor(es):
Dias, Rodrigo G. [1] ; Alves, Maria-Janieire N. N. ; Pereira, Alexandre C. ; Rondon, Maria Urbana P. B. ; dos Santos, Marcelo R. ; Krieger, Jose E. ; Krieger, Marta H. [1] ; Negrao, Carlos E. [2, 3]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, LabCardio, Campinas, SP - Brazil
[2] Univ Sao Paulo, Sch Phys Educ & Sport, Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Med, InCor, Heart Inst, Unidade Reabilitacao Cardiovasc & Fisiol Exercici, BR-05403000 Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Physiological Genomics; v. 37, n. 2, p. 99-107, APR 2009.
Citações Web of Science: 24
Resumo

Dias RG, Alves MJ, Pereira AC, Rondon MU, dos Santos MR, Krieger JE, Krieger MH, Negrao CE. Glu298Asp eNOS gene polymorphism causes attenuation in nonexercising muscle vasodilatation. Physiol Genomics 37: 99-107, 2009. First published January 21, 2009; doi:10.1152/physiolgenomics.90368.2008.-The influence of Glu298Asp endothelial nitric oxide synthase (eNOS) polymorphism in exercise-induced reflex muscle vasodilatation is unknown. We hypothesized that nonexercising forearm blood flow (FBF) responses during handgrip isometric exercise would be attenuated in individuals carrying the Asp298 allele. In addition, these responses would be mediated by reduced eNOS function and NO-mediated vasodilatation or sympathetic vasoconstriction. From 287 volunteers previously genotyped, we selected 33 healthy individuals to represent three genotypes: Glu/Glu {[}n = 15, age 43 +/- 3 yr, body mass index (BMI) 22.9 +/- 0.3 kg/m(2)], Glu/Asp (n = 9, age 41 +/- 3 yr, BMI 23.7 +/- 1.0 kg/m(2)), and Asp/Asp (n = 9, age 40 +/- 4 yr, BMI 23.5 +/- 0.9 kg/m(2)). Heart rate (HR), mean blood pressure (MBP), and FBF (plethysmography) were recorded for 3 min at baseline and 3 min during isometric handgrip exercise. Baseline HR, MBP, FBF, and forearm vascular conductance (FVC) were similar among genotypes. FVC responses to exercise were significantly lower in Asp/Asp when compared with Glu/Asp and Glu/Glu (Delta = 0.07 +/- 0.14 vs. 0.64 +/- 0.20 and 0.57 +/- 0.09 units, respectively; P = 0.002). Further studies showed that intra-arterial infusion of N(G)-monomethyl-L-arginine (L-NMMA) did not change FVC responses to exercise in Asp/Asp, but significantly reduced FVC in Glu/Glu (Delta = 0.79 +/- 0.14 vs. 0.14 +/- 0.09 units). Thus the differences between Glu/Glu and Asp/Asp were no longer observed (P = 0.62). L-NMMA + phentolamine increased similarly FVC responses to exercise in Glu/Glu and Asp/Asp (P = 0.43). MBP and muscle sympathetic nerve activity increased significant and similarly throughout experimental protocols in Glu/Glu and Asp/Asp. Individuals who are homozygous for the Asp298 allele of the eNOS enzyme have attenuated nonexercising muscle vasodilatation in response to exercise. This genotype difference is due to reduced eNOS function and NO-mediated vasodilatation, but not sympathetic vasoconstriction. (AU)

Processo FAPESP: 04/14292-4 - Análise da atividade nervosa simpática periférica e da vasodilatação endotélio-dependente em indivíduos portadores do polimorfismo Glu298Asp da eNOS
Beneficiário:Rodrigo Gonçalves Dias
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 05/59740-7 - Exercício físico e controle autonômico na fisiopatologia cardiovascular
Beneficiário:Carlos Eduardo Negrão
Linha de fomento: Auxílio à Pesquisa - Temático