Effects of Er:YAG laser irradiation and manipulation treatments on dentin components, part 1: Fourier transform-Raman study

Autor(es)

Silva Soares, Luis Eduardo ^[1] ; do Espirito Santo, Ana Maria ; Brugnera Junior, Aldo ^[2] ; Aparecida Zanin, Fatima Antonia ^[2] ; da Silva Carvalho, Carolina ; de Oliveira, Rodrigo ; Martin, Airton Abrahao

Afiliação do(s) autor(es)

^[1] Univ Vale Paraiba, Inst Pesquisa & Desenvolvimento, IP&D, LEVB, Sch Dent, Dent Mat & Operat Dent Dept, BR-12244000 Sao Jose Dos Campos, SP - Brazil

^[2] Univ Vale Paraiba, Inst Pesquisa & Desenvolvimento, IP&D, Dent Laser Ctr, BR-12244000 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biomedical Optics; v. 14, n. 2 MAR-APR 2009.
• Citações Web of Science: 8

Resumo

The effects of laser etching, decontamination, and storage treatments on dentin components were studied using Fourier transform (FT)-Raman spectroscopy. Thirty bovine incisors were prepared to expose the dentin surface and then divided in two main groups based upon the decontamination process and storage procedure: autoclaved (group A, n = 15) or stored in thymol aqueous solution (group B, n = 15). The surfaces of the dentin slices were schematically divided into four areas, with each one corresponding to a treatment subgroup. The specimens were either etched with phosphoric acid (control subgroup) or irradiated with erbium-doped yttrium-aluminum-garnet (Er:YAG) laser (subgroups: I-80 mJ, II-120 mJ, and III-180 mJ, and total energy of 12 J). Samples were analyzed by FT-Raman spectroscopy; we collected three spectra for each area (before and after treatment). The integrated areas of five Raman peaks were calculated to yield average spectra. The areas of the peaks associated with phosphate content (P < 0.001), type I collagen, and organic C-H bonds (P < 0.05) were reduced significantly in group A (control). Analyses of samples irradiated with reduced laser energies did not show significant changes in the dentin components. These results suggest that thymol storage treatment is advised for in vitro study; furthermore, 12 J of Er:YAG laser energy does not affect dentin components. (C) 2009 Society of Photo-Optical Instrumentation Engineers. {[}DOI: 10.1117/1.3103285] (AU)

• Processo FAPESP: 01/14384-8 - Estudo de células neoplásicas por espectroscopia de infravermelho próximo
• Beneficiário: Airton Abrahao Martin
• Modalidade de apoio: Auxílio à Pesquisa - Regular