Evaluation of different protocols for anatomical studies in Parmeliaceae (lichenized Ascomycota)

Autor(es)

Barbosa, Suzana Bissacot ^[1] ; Marcelli, Marcelo Pinto ^[2] ; Machado, Silvia Rodrigues ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Inst Biociencias, Dept Bot, BR-18618000 Botucatu, SP - Brazil

^[2] Inst Bot, Secao Micol & Liquenol, BR-01061970 Sao Paulo - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: Micron; v. 40, n. 2, p. 218-225, FEB 2009.
• Citações Web of Science: 8

Resumo

Anatomical information on most lichen species, including Parmeliaceae species, is scarce and superficial. This is partially due to the technical difficulties found during the preparation of samples and sections suitable for optical microscopy analysis. There is a lack of pictures of anatomical sections as well as detailed anatomical protocols related to the sample processing made by specialized plant anatomists in literature. This work aimed to look for a standardized histological technique and to develop a protocol for histological studies of foliose Parmeliaceae under light microscopy. Four common pantropical species of Parmeliaceae, abundant in the Brazilian cerrado vegetation, were processed in several ways in relation to fixing and inclusion media, sectioning thicknesses, and staining, including both fresh and 2-year-old herbarium specimens. The best technique found for anatomical studies of Parmeliaceae under light microscopy is represented by 2 or 3 mu m thick sections of fresh or herbarium samples, desiccated and fixed in FAA 50, infiltrated and embedded in glycol-methacrylate resin (Leica), and stained by Toluidine Blue or Methylene Blue. (c) 2008 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 00/12469-3 - Estudos morfológicos, anatômicos, histoquímicos e ultra-estruturais em plantas do Cerrado (Senso lato) do estado de São Paulo
• Beneficiário: Silvia Rodrigues Machado
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático