Hepatotoxin microcystin-LR extraction optimization

Autor(es)

Silva-Stenico, Maria Estela ^[1] ; Cantusio Neto, Romeu ^[2] ; Alves, Ivanio R. ^[2] ; Moraes, Luiz Alberto B. ^[3] ; Shishido, Tania K. ^[1] ; Fiore, Marli Fatima ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, CENA, BR-13400970 Piracicaba, SP - Brazil

^[2] Sociedade Abastecimento Agua & Saneamento SANASA, Cent Lab, BR-13045610 Campinas, SP - Brazil

^[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 20, n. 3, p. 535-542, 2009.
• Citações Web of Science: 20

Resumo

Several cyanobacterial genera produce toxic secondary metabolites, the most well-known of which are the hepatotoxic microcystins (MCYSTs). Microcystin analyses in drinking water are a requirement of the Health Ministry (Regulation 518/2004) in Brazil, but this regulation does not establish which extraction and analytical method should be used; toxin quantification is usually carried out by ELISA (enzyme-linked immunosorbent assay) or HPLC (high performance liquid chromatography), the efficiency of which depends on the extraction method used. In this work a simple, fast and cheap method of extraction was developed for the isolation and identification of MCYSTs. For this, the strain Microcystis aeruginosa NPLJ-4, reported to be a MCYST-LR producer, was selected. Eight different treatments were tested to determine the best MCYST extraction. Samples were applied in LC-MS (liquid chromatography-mass spectrometry), ELISA and Q-TOF (quadrupole time-of-flight). The most efficient extraction was achieved by sonicating samples diluted in water. The proposed method permits rapid sample processing, and establishes an extraction method for both the analysis and identification of MCYST-LR and other variants. (AU)

• Processo FAPESP: 04/16042-5 - Nanotecnologia: observação das estruturas formadas pela interação entre peptídeos não-ribossômicos, mutantes de metalotioneínas e lotioneinas e metais
• Beneficiário: Maria Estela Stenico
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 04/16041-9 - Selecao de cianobacterias produtoras de peptideos nao ribossomicos com atividade antimicrobiana.
• Beneficiário: Tânia Keiko Shishido
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 05/56303-5 - Caracterizacao molecular de cianobacterias brasileiras e selecao de linhagens produtoras de peptideos nao ribossomicos.
• Beneficiário: Marli de Fátima Fiore
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 03/12529-4 - Nanotecnologia: fabricação de nanoestruturas e estruturas supramoleculares em meio líquido: observação das estruturas formadas e investigação dos processos de formação de nanoestruturas
• Beneficiário: Omar Teschke
• Modalidade de apoio: Auxílio à Pesquisa - Temático