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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Subversion of antimicrobial calprotectin (S100A8/S100A9 complex) in the cytoplasm of TR146 epithelial cells after invasion by Listeria monocytogenes

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Autor(es):
Zaia, A. A. [1, 2, 3] ; Sappington, K. J. [1, 2] ; Nisapakultorn, K. [1, 2] ; Chazin, W. J. [4] ; Dietrich, E. A. [1, 2] ; Ross, K. F. [1, 2] ; Herzberg, M. C. [1, 2]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Minnesota, Sch Dent, Dept Diagnost & Biol Sci, Minneapolis, MN 55455 - USA
[2] Minneapolis VA Med Ctr, Mucosal & Vaccine Res Ctr, Minneapolis, MN - USA
[3] Univ Estadual Campinas, Sch Dent, Dept Restorat Sci, Campinas - Brazil
[4] Vanderbilt Univ, Dept Biochem, Struct Biol Ctr, Nashville, TN 37232 - USA
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: MUCOSAL IMMUNOLOGY; v. 2, n. 1, p. 43-53, JAN 2009.
Citações Web of Science: 29
Resumo

Expressed by squamous mucosal keratinocytes, calprotectin is a complex of two EF-hand calcium-binding proteins of the S100 subfamily (S100A8 and S100A9) with significant antimicrobial activity. Calprotectin-expressing cells resist invasion by Porphyromonas gingivalis, Listeria monocytogenes, and Salmonella enterica serovar Typhimurium ( S. typhimurium). To understand the interactions between calprotectin and invasive bacteria, we studied the distribution of calprotectin in the cytoplasm of TR146 epithelial cells. In response to L. monocytogenes, calprotectin mobilized from a diffuse cytoplasmic distribution to a filamentous pattern and colocalized with the microtubule network. Listeria more frequently invaded cells with mobilized calprotectin. Calprotectin mobilization was listeriolysin O-dependent and required calcium ( extracellular and intracellular) and an intact microtubule network. In the presence of preformed microtubules in vitro, the anti-Listeria activity of calprotectin was abrogated. To facilitate intraepithelial survival, therefore, Listeria mobilizes calprotectin to colocalize with cytoplasmic microtubules, subverting anti-Listeria activity and autonomous cellular immunity. (AU)

Processo FAPESP: 99/10079-4 - Análise da expressão e regulação "in situ" da calprotectin em gengivas saudáveis e durante doença periodontal
Beneficiário:Alexandre Augusto Zaia
Linha de fomento: Bolsas no Exterior - Pesquisa