In Vitro Antibacterial Activity of Adhesive Systems on Streptococcus Mutans

Autor(es)

Paradella, Thais Cachute ^[1] ; Koga-Ito, Cristiane Yumi ^[2] ; Cardoso Jorge, Antonio Olavo ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Dept Oral Biopathol, UNESP, Sao Jose Campos Dent Sch, Sao Jose Dos Campos, SP - Brazil

^[2] Sao Paulo State Univ, Dept Oral Microbiol, UNESP, Sao Jose Campos Dent Sch, Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Adhesive Dentistry; v. 11, n. 2, p. 95-99, 2009.
• Citações Web of Science: 16

Resumo

Purpose: To evaluate the antibacterial activity of three adhesive systems - Prime \& Bond 2.1 (PB), Clearfil SE Bond (CS) and One Up Bond F (OU) - on Streptococcus mutans in vitro. Materials and Methods: Adherence and agar disk-diffusion tests were performed. For the adherence testing, 40 human enamel specimens (4 mm(2)) were sterilized and the adhesive sytems were applied (n = 10). The control group did not receive the application of any adhesive system. Specimens were immersed in brain heart infusion broth (BHI) inoculated with S. mutans standardized suspension (106 cells/ml) for 48 h at 37 degrees C and 5% CO(2). The number of S. mutans cells adhered to each specimen was evaluated by the plating method on BHI agar. For agar disk-diffusion testing, adhesive disks and disks soaked in distilled water (negative control) or 0.2% chlorexidine (positive control) were incubated with S. mutans for 48 h. The diameters of the zones of bacterial inhibition were measured. Adherence data were transformed in logarithms of base 10 (log(10)). Data were submitted to Kruskal-Wallis and Student-Neuman-Keuls tests at the 5% level of significance. Results: The results of the adherence test showed that One Up Bond F (OU) and Clearfil SE Bond (CS) did not differ significantly from one another, but allowed significantly less adherence than Prime \& Bond 2.1 (PB) and control {[}mean log(10) (standard deviation) values: PB 6.10 (0.19); CS primer 4.55 (0.98); OU 4.65 (0.54); control group 6.34 (0.27)]. The disk-diffusion test showed no significant difference between OU (diameter in mm: 3.02 +/- 0.13) and CS (3.0 +/- 0.12), but both were significantly more effective in inhibiting bacteria] growth than PB (1.0 +/- 0.10). Conclusion: The self-etching systems Clearfil SE Bond and One Up Bond F presented a greater inhibitory effect against S. mutans, also in terms of adherence, than did the conventional system, Prime \& Bond 2.1. (AU)

• Processo FAPESP: 06/52513-8 - Estudo da aderencia, desafio cariogenico com streptococcus mutans e ciclagem de ph em materiais restauradores: analise por microscopia de luz polarizada e espectroscopia por dispersao de raios-x (eds)
• Beneficiário: Antonio Olavo Cardoso Jorge
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/58458-6 - Desafio cariogenico com streptococcus mutans e ciclagem de ph em materiais restauradores: analise por microscopia de luz polarizada e espectroscopia por dispersao de raios-x (eds).
• Beneficiário: Thaís Cachuté Paradella
• Modalidade de apoio: Bolsas no Brasil - Doutorado