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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Differential expression of myogenic regulatory factor MyoD in pacu skeletal muscle (Piaractus mesopotamicus Holmberg 1887: Serrasalminae, Characidae, Teleostei) during juvenile and adult growth phases

Texto completo
Autor(es):
Alves de Almeida, Fernanda Losi [1, 2] ; Carvalho, Robson Francisco [1] ; Pinhal, Danillo [1] ; Padovani, Carlos Roberto [3] ; Martins, Cesar [1] ; Dal Pai-Silva, Maeli [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, Inst Biosci, Dept Morphol, BR-18618000 Sao Paulo - Brazil
[2] Univ Estadual Campinas, Dept Cellular Biol, Inst Biol, BR-13084971 Sao Paulo - Brazil
[3] UNESP, Dept Biostat, BR-18618000 Botucatu, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo de Revisão
Fonte: Micron; v. 39, n. 8, p. 1306-1311, DEC 2008.
Citações Web of Science: 25
Resumo

Skeletal muscle is the edible part of the fish. It grows by hypertrophy and hyperplasia, events regulated by differential expression of myogenic regulatory factors (MRFs). The study of muscle growth mechanisms in fish is very important in fish farming development. Pacu (Piaractus mesopotamicus) is one of the most important food species farmed in Brazil and has been extensively used in Brazilian aquaculture programs. The aim of this study was to analyze hyperplasia and hypertrophy and the MRF MyoD expression pattern in skeletal muscle of pacu (P. mesopotamicus) during juvenile and adult growth stages. Juvenile (n = 5) and adult (n = 5) fish were anaesthetized, sacrificed, and weight (g) and total length (cm) determined. White dorsal region muscle samples were collected and immersed in liquid nitrogen. Transverse sections (10 mu m thick) were stained with Haematoxilin-Eosin (HE) for morphological and morphometric analysis. Smallest fiber diameter from 100 muscle fibers per animal was calculated in each growth phase. These fibers were grouped into three classes (<20, 20-50, and >50 mu m) to evaluate hypertrophy and hyperplasia in white skeletal muscle. MyoD gene expression was determined by semi-quantitative RTPCR. PCR products were cloned and sequenced. juvenile and adult pacu skeletal muscle had similar morphology. The large number of <20 mu m diameter muscle fibers observed in juvenile fish confirms active hyperplasia. In adult fish, most fibers were over 50 mu m diameter and denote more intense muscle fiber hypertrophy. The MyoD mRNA level in juveniles was higher than in adults. A consensus partial sequence for MyoD gene (338 base pairs) was obtained. The Pacu MyoD nucleotide sequence displayed high similarity among several vertebrates, including teleosts. The differential MyoD gene expression observed in pacu white muscle is possibly related to differences in growth patterns during the phases analyzed, with hyperplasia predominant in juveniles and hypertrophy in adult fish. These results should provide a foundation for understanding the molecular control of skeletal muscle growth in economically important Brazilian species, with a view to improving production quality. (C) 2008 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 05/56587-3 - Expressão dos fatores de regulação miogênica no tecido muscular esquelético do pacu (Piaractus mesopotamicus) Holmberg 1887: Serrasalminae, Characidae, Teleostei)
Beneficiário:Maeli Dal Pai
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 04/12756-3 - Expressão dos fatores de regulação miogênica no tecido muscular esquelético do pacu (Piaractus mesopotamicus Holmberg 1887: Serrasalminae, Characidae, Teleostei)
Beneficiário:Fernanda Losi Alves de Almeida
Linha de fomento: Bolsas no Brasil - Mestrado