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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

MyoD, myogenin and proliferating cell nuclear antigen expression in growing Nile tilapia (Oreochromis niloticus L.)

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Autor(es):
Aguiar, Danilo Henrique [1, 2, 3] ; Bock, Claudio [4] ; Padovani, Carlos Roberto [5] ; Dal Pai-Silva, Maeli [1, 2]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] UNESP, Dept Morphol, Inst Biosci, BR-18618000 Botucatu, SP - Brazil
[2] CAUNESP, Botucatu, SP - Brazil
[3] Univ Estadual Campinas, Dept Cellular Biol, Inst Biol, Campinas - Brazil
[4] IBAMA, Ctr Res & Training Aquaculture, CEPTA, Pirassununga, SP - Brazil
[5] UNESP, Inst Biosci, Dept Biostat, BR-18618000 Botucatu, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: AQUACULTURE RESEARCH; v. 39, n. 15, p. 1673-1679, NOV 4 2008.
Citações Web of Science: 11
Resumo

In the present study, immunohistochemical and morphometric analysis was used to characterize variations in muscle growth performance during muscle fibre recruitment and hypertrophy. As in fisheries, fish were classified into four age stages: alevin 35 days (0.65 +/- 0.08 g); juvenile 60 days (13.67 +/- 1.35 g); adult 90 days (73.18 +/- 4.70 g) and adult 190 days (349.76 +/- 34.62 g). The number of nuclei expressing MyoD and myogenin was similar in alevin, juvenile and adult 90 days; however, in adult 190 days, the number of nuclei expressing myogenin was higher than the number expressing MyoD. The number of proliferating cell nuclear antigen-stained nuclei in each stage was higher than MyoD and myogenin staining with peaks in alevin and adult 90 days. These data suggest that growth per se stimulated cellular proliferation and nuclei accretion of Nile tilapia muscle fibres in alevin, juvenile and adult 90 days. Muscle fibre differentiation was more pronounced in adult 190 days. (AU)

Processo FAPESP: 04/13286-0 - Expressão de fatores de regulação miogênica (MRFs) e da miosina de cadeia pesada (MHC) no músculo estriado esquelético da Tilápia do Nilo (Oreochromis niloticus) durante o crescimento
Beneficiário:Danilo Henrique Aguiar
Modalidade de apoio: Bolsas no Brasil - Doutorado