Evaluation of the Binding Properties of Maghemite Nanoparticle Surface-Coated with Meso-2-3-Dimercaptosuccinic Acid to Serum Albumin

Autor(es)

Simoni, Andreza R. ^[1] ; Garcia, Mnica P. ^[2] ; Azevedo, Ricardo B. ^[2] ; Chaves, Sacha Braun ^[2] ; Lacava, Z. G. M. ^[2] ; Lima, E. C. D. ^[3] ; Morais, P. C. ^[4] ; Tedesco, A. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, FFCLRP USP, BR-14040901 Ribeirao Preto - Brazil

^[2] Univ Brasilia, Inst Ciencias Biol, BR-70910900 Brasilia, DF - Brazil

^[3] Univ Fed Goias, Inst Quim, BR-74001970 Goiania, Go - Brazil

^[4] Univ Brasilia, Inst Fis Nucl Fis Aplicada, BR-70910900 Brasilia, DF - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Nanoscience and Nanotechnology; v. 8, n. 11, p. 5813-5817, NOV 2008.
• Citações Web of Science: 5

Resumo

In this study the interaction between magnetic nanoparticles (MNPs) surface-coated with meso-2,3-dimercaptosuccinic acid (DMSA) with both bovine serum albumin (BSA) and human serum albumin (HSA) was investigated. The binding of the MNP-DMSA was probed by the fluorescence quenching of the BSA and HSA tryptophan residue. Magnetic resonance and light microscopy analyses were carried out in in vivo tests using female Swiss mice. The binding constants (K(b)) and the complex stoichiometries (n) indicate that MNP-DMSA/BSA and MNP-DMSA/HSA complexes have low association profiles. After five minutes following intravenous injection of MNP-DMSA into mice's blood stream we found the lung firstly target by the MNP-DMSA, followed by the liver in a latter stage. This finding suggests that the nanoparticle's DMSA-coating process probably hides the thiol group, through which albumin usually binds. This indicates that biocompatible MNP-DMSA is a very promising material system to be used as a drug delivery system (DDS), primarily for lung cancer treatment. (AU)

• Processo FAPESP: 04/00860-0 - Estudos de silício-naftalo ftalocianina como agente potencialmente ativo na terapia fotodinâmica de neoplasias cutâneas e sistêmicas
• Beneficiário: Andreza Ribeiro Simioni
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto