Antimicrobial activity of Rheedia brasiliensis and 7-epiclusianone against Streptococcus mutans

Autor(es)

Almeida, L. S. B. ^[1] ; Murata, R. M. ^[1] ; Yatsuda, R. ^[2] ; Dos Santos, M. H. ^[3] ; Nagem, T. J. ^[4] ; Alencar, S. M. ^[5] ; Koo, H. ^[6] ; Rosalen, P. L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Dent Piracicaba, Dept Physiol Sci, Campinas, SP - Brazil

^[2] Univ Fed Bahia, Ctr Technol Hlth, Vitoria Da Conquista, BA - Brazil

^[3] Univ Fed Alfenas, Lab Phytochem & Med Chem, Alfenas, MG - Brazil

^[4] Univ Fed Ouro Preto, Dept Chem ICEB, Ouro Preto, MG - Brazil

^[5] Univ Sao Paulo, Escola Super Agr Luiz Queiroz, Dept Agri Food Ind Food & Nutr, Piracicaba, SP - Brazil

^[6] Univ Rochester, Med Ctr, Eastman Dept Dent, Ctr Oral Biol, Rochester, NY 14642 - USA

• Tipo de documento: Artigo Científico
• Fonte: Phytomedicine; v. 15, n. 10, p. 886-891, OCT 2008.
• Citações Web of Science: 38

Resumo

This in vitro study evaluated the antimicrobial activity of extracts obtained from Rheedia brasiliensis fruit (bacupari) and its bioactive compound against Streptococcus mutans. Hexane, ethyl-acetate and ethanolic extracts obtained (concentrations ranging from 6.25 to 800 mu g/ml) were tested against S. mutans UA159 through MIC/MBC assays. S. mutans 5-days-old biofilms were treated with the active extracts (100 x MIC) for 0, 1, 2, 3 and 4 h (time-kill) and plated for colony counting (CFU/ml). Active extracts were submitted to exploratory chemical analyses so as to isolate and identify the bioactive compound using spectroscopic methods. The bioactive compound (concentrations ranging from 0.625 to 80 mu g/ml) was then tested through MIC/MBC assays. Peel and seed hexane extracts showed antimicrobial activity against planktonic cells at low concentrations and were thus selected for the time kill test. These hexane extracts reduced S. mutans biofilm viability after 4 h, certifying of the bioactive compound presence. The bioactive compound identified was the polyprenylated benzophenone 7-epiclusianone, which showed a good antimicrobial activity at low concentrations (MIC: 1.25-2.5 mu g/ml; MBC: 10-20 mu g/ml). The results indicated that 7-epiclusianone may be used as a new agent to control S. mutans biofilms; however, more studies are needed to further elucidate the mechanisms of action and the anticariogenic potential of such compound found in R. brasiliensis. (C) 2008 Elsevier GmbH. All rights reserved. (AU)

• Processo FAPESP: 06/56379-4 - Potencial antimicrobiano de um novo composto poliprenilado extraido da rheedia brasiliensis e sua influencia na expressao proteica de biofilmes de streptococcus mutans.
• Beneficiário: Maria Cristina Volpato
• Modalidade de apoio: Auxílio à Pesquisa - Regular