Shannon's entropy and fractal dimension provide an objective account of bone tissue organization during calvarial bone regeneration

Autor(es)

Rocha, Lenaldo B. ^[1] ; Adam, Randall L. ^[2] ; Leite, Neucimar J. ^[3] ; Metze, Konradin ^[2] ; Rossi, Marcos A. ^[1]

Afiliação do(s) autor(es)

^[1] USP, Fac Med Ribeirao Preto, Dept Pathol, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Estadual Campinas, Fac Med, Dept Pathol, BR-13081970 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Inst Comp, Dept Informat Syst, BR-13084971 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROSCOPY RESEARCH AND TECHNIQUE; v. 71, n. 8, p. 619-625, AUG 2008.
• Citações Web of Science: 12

Resumo

The regeneration of compact bone involves the deposition of a poorly organized connective tissue template that remodels into compact lamellar bone. An objective description of this process is difficult because classical histomorphometry is unable to correctly characterize qualitative changes in tissue complexity. In this study, we demonstrated the use of two distinct methods of image texture analysis, the Shannon's entropy {[}standard error (SE)], and the fractal dimension (FD) to characterize the formation and remodeling of newly formed compact bone within two different polyanionic collagen-elastin matrices. The matrices were implanted in defects created into parietal bones of rats. The SE and FD were calculated for histological images of the experimental groups collected 3, 7, 15, 30, 60, and 365 days postsurgery and for the original bone only at day 365. Results showed that the SE and the FD initially increased and then diminished for all groups from clay 3 to (lay 365 approaching the values of the original bone. These results are consistent with poor tissue organization during early osteogenesis that remodels into an organized lamellar Structure, showing that these methods can be valuable tools to describe bone tissue remodeling during the regeneration process of compact bones. (AU)

• Processo FAPESP: 01/05634-0 - Reparo de defeitos osseos de tamanho critico com matrizes de colageno anionico.
• Beneficiário: Lenaldo Branco Rocha
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 01/14059-0 - Engenharia de tecido osseo: reacao inflamatoria, diferenciacao osteblastica e proliferacao vascular em matrizes de colageno anionico.
• Beneficiário: Marcos Antonio Rossi
• Modalidade de apoio: Auxílio à Pesquisa - Regular