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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Dipeptide Synthesis in Biphasic Medium: Evaluating the use of Commercial Porcine Pancreatic Lipase Preparations and the Involvement of Contaminant Proteases

Texto completo
Liria, Cleber W. [1] ; Romagna, Carolina D. [1] ; Rodovalho, Nicolas N. [1] ; Marana, Sandro R. [1] ; Miranda, M. Teresa M. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Bioquim, Inst Quim, BR-05513970 Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Journal of the Brazilian Chemical Society; v. 19, n. 8, p. 1574-1581, 2008.
Citações Web of Science: 8

Dipeptide syntheses starting from Ac-L-Tyr-OEt or Z-L-X-OMe (X: Asp, Tyr, Phe, Arg, Lys or Thr) and glycine amide in biphasic reaction media were achieved using two commercially available porcine pancreatic lipase (PPL) preparations (crude (cPPL) and purified PPL (pPPL)). Under the mild conditions employed, alpha-chymotrypsin, a pancreatic protease that also presents esterase activity, catalyzed Ac-L-Tyr-Gly-NH(2) synthesis with high productivity. Product hydrolysis also occurred in most of the syntheses studied. Polyacrylamide gel electrophoresis, enzymatic assays employing specific chromogenic substrates and size-exclusion chromatography revealed that cPPL and pPPL contain contaminant proteases and, therefore, exhibit esterase and amidase activities. Overall, these data indicate that those contaminants may be the main catalysts of peptide bond synthesis when N(alpha)-blocked-L-amino acid esters and the commercial PPL preparations are used. On the other hand, such data do not contest the possibility of using such enzyme preparations as an inexpensive source of catalysts for dipeptide synthesis under soft conditions. (AU)

Processo FAPESP: 00/05410-2 - Diferentes aspectos da síntese de peptpídeos longos e de sequências difíceis: parte II
Beneficiário:Maria Teresa Machini
Linha de fomento: Auxílio à Pesquisa - Regular