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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The use of fluorescein for labeling genomic probes in the checkerboard DNA-DNA hybridization method

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Autor(es):
do Nascimento, Cassio [1] ; Santos Barbosa, Rodrigo Edson [1] ; Mardegan Issa, Joao Paulo [1] ; Watanabe, Evandro [2] ; Ito, Izabel Yoko [2] ; Monesi, Nadia [2] ; de Albuquerque Junior, Rubens Ferreira [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Mat Dentarios & Protese, Fac Odontol Ribeirao Preto, BR-14040904 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: MICROBIOLOGICAL RESEARCH; v. 163, n. 4, p. 403-407, 2008.
Citações Web of Science: 7
Resumo

Molecular methods that permit the simultaneous detection and quantification of a large number of microbial species are currently employed in the evaluation of complex ecosystems. The checkerboard DNA-DNA hybridization technique enables the simultaneous identification of distinct bacterial. species in a large number of dental samples. The original technique employed digoxigenin-labeled whole genomic DNA probes which were detected by chemiluminescence. In this study, we present an alternative protocol for labeling and detecting whole genomic DNA probes in the Checkerboard DNA-DNA hybridization method. Whole genomic DNA was extracted from five bacterial species and labeled with fluorescein. The fluorescein labeled whole genomic DNA probes were hybridized against whole genomic DNA or subgingival plaque samples in a checkerboard hybridization format, followed by chemiluminescent detection. Our results reveal that fluorescein is a viable and adequate alternative labeling reagent to be employed in the checkerboard DNA-DNA hybridization technique. (c) 2007 Elsevier GmbH. All rights reserved. (AU)

Processo FAPESP: 03/04585-1 - Avaliacao da contaminacao peri e intra-implantar e de proteses rosqueadas sobre implantes dentarios.
Beneficiário:Rubens Ferreira de Albuquerque Junior
Modalidade de apoio: Auxílio à Pesquisa - Regular