Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Acute and short-term insulin-induced molecular adaptations of GLUT2 gene expression in the renal cortex of diabetic rats

Texto completo
Autor(es):
Freitas, Helayne Soares [1] ; Schaan, Beatriz D’Agord ; Seraphim, Patricia Monteiro ; Nunes, Maria Tereza ; Machado, Ubiratan Fabres
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Universidade de São Paulo (USP). Instituto de Ciências Biomédicas. Departamento de Fisiologia e Biofísica - Brasil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Molecular and Cellular Endocrinology; v. 237, n. 1/2, p. 49-57, Jun. 2005.
Área do conhecimento: Ciências Biológicas - Fisiologia
Assunto(s):Túbulos renais proximais   Nefropatias diabéticas   Expressão gênica
Resumo

Increased GLUT2 gene expression in the renal proximal tubule of diabetic rats is an adaptive condition, which may be important in the diabetic nephropathy development. We investigated the effects of insulin treatment upon the renal GLUT2 overexpression of diabetic rats. Acute treatment, surprisingly, induced a rapid further increase in GLUT2 mRNA content. Twelve hours after insulin injection, GLUT2 mRNA was twice the value of saline-injected rats (P < 0.001), when GLUT2 protein remained unchanged. In response to short-term treatment, both GLUT2 mRNA and protein were increased in 1-day treated rats (P < 0.05 versus saline-injected), decreasing after that, and reaching, within 6 days, values close to those of non-diabetic rats. Concluding, insulin treatment induced: initially, an additional upregulation of GLUT2 gene expression, involving posttranscriptional modulation; thereafter, downregulation of GLUT2 expression, which returns to non-diabetic levels. The former may be related to increased insulin concentration, the latter may be due to glycemic control. (AU)

Processo FAPESP: 02/07384-4 - Regulação da expressão gênica dos transportadores de glicose no diabetes mellitus: papel na fisiopatologia e potencialidades preventivas e terapêuticas
Beneficiário:Ubiratan Fabres Machado
Linha de fomento: Auxílio à Pesquisa - Temático