Expression of New Red Cell-Related Genes in Erythroid Differentiation

Autor(es)

De Andrade, Tiago ^[1] ; Moreira, Luciana ^[2] ; Duarte, Adriana ^[2] ; Lanaro, Carolina ^[2] ; De Albuquerque, Dulcineia ^[2] ; Saad, Sara ^[2] ; Costa, Fernando ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Alagoas, Arapiraca, AL - Brazil

^[2] Univ Estadual Campinas, Fac Med Sci, Hemoctr, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOCHEMICAL GENETICS; v. 48, n. 1-2, p. 164-171, FEB 2010.
• Citações Web of Science: 4

Resumo

Using a suppression subtractive hybridization method, we have previously identified genes differentially expressed in erythroid cells heterozygous for large deletions in beta-like globin cluster. Herein, we investigated the expression of four newly detected red cell-related genes in erythroid differentiation. ARID1B and TSPYL1, genes with chromatin remodeling properties, presented similar patterns of expression with an upregulation after erythropoietin (EPO) addition, similar to previous data found in reticulocytes. ZHX2, a transcriptional repressor, was downregulated, and a redoxin-related gene, SH3BGRL2, had higher levels of expression on differentiation. These are the first investigations of these newly described genes in erythroid differentiation and demonstrate that the expression of these genes is affected by EPO stimulation. These genes may participate in globin regulation and may be important in the normal physiology of erythrocytes. (AU)

• Processo FAPESP: 02/03396-8 - Mecanismos reguladores da produção de hemoglobina fetal: análise da expressão gênica na persistência hereditária de hemoglobina fetal e nas delta-beta talassemias, e avaliação funcional da região R/PYR na expressão dos genes gama
• Beneficiário: Tiago Gomes de Andrade
• Modalidade de apoio: Bolsas no Brasil - Doutorado