| Texto completo | |
| Autor(es): |
Teixeira Gomes, Regina Celia
[1]
;
Verna, Carina
[1]
;
Nader, Helena Bonciani
[2]
;
Simoes, Ricardo dos Santos
[3]
;
Dreyfuss, Juliana L.
[2]
;
Maciel Martins, Joao Roberto
[3, 2, 4]
;
Baracat, Edmund C.
[3]
;
Simoes, Manuel de Jesus
[1]
;
Soares, Jr., Jose Maria
[3]
Número total de Autores: 9
|
| Afiliação do(s) autor(es): | [1] Univ Fed Sao Paulo, Dept Morphol Histol & Struct Biol, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Dept Biochem, Div Mol Biol, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, Dept Med, Div Endocrinol & Metab, Sao Paulo - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Fertility and Sterility; v. 92, n. 2, p. 785-792, AUG 2009. |
| Citações Web of Science: | 17 |
| Resumo | |
Objective: To quantify and study the immunoexpression of hyaluronic acid (HA) in the uterine horns of the mouse throughout the estrous cycle phases. Design: Experimental study using an ELISA-like fluorometric assay to quantify HA and an avidin-biotin immunoperoxidase method using biotinylated hyaluronan-binding protein for histochemical studies. Setting: University-based laboratory. Animal(s): Forty regularly cycling adult female mice were divided into four groups according to the diagnosed phase of the cycle: proestrus, estrus, metaestrus, and diestrus. Intervention(s): None. Main outcome measure(s): Histologic samples of the uterine horns. Immunohistochemical reaction was evaluated by detection of HA and CD44 distribution within the uterine horn. Tissue HA content was determined through an ELISA-like fluorometric assay with the same hyaluronan-binding protein and with europium-labeled streptavidin. Result(s): The immunohistochemical HA and CD44 reactions were most intense during diestrus, mainly below the luminal epithelium. HA was strongly stained in the connective tissue near the myometrium layer during metaestrus. The biochemical data showed that the highest concentration of HA in uterine horns occurred during diestrus (4053.0 +/- 651.4 ng/g dry tissue) compared with other phases. Conclusion(s): Our data show that the expression of HA in mouse uterine horns is highest during the diestrous phase. The fluctuations of HA in the mouse uterus during the estrous phase may be related to the varying estrogen and P levels during the cycle and may be important as far as embryo implantation is concerned. (Fertil Steril (R) 2009;92:785-92. (c) 2009 by American Society for Reproductive Medicine.) (AU) | |
| Processo FAPESP: | 06/50639-4 - Efeitos da hiperprolactinemia no utero de camundongos durante as fases do ciclo estral. |
| Beneficiário: | José Maria Soares Junior |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |