Callus, shoot and hairy root formation in vitro as affected by the sensitivity to auxin and ethylene in tomato mutants

Autor(es)

Lima, Joni Esrom ^[1] ; Benedito, Vagner Augusto ^[1] ; Figueira, Antonio ^[1] ; Pereira Peres, Lazaro Eustaquio ^[2]

Afiliação do(s) autor(es)

^[1] USP, CENA, BR-13400970 Piracicaba, SP - Brazil

^[2] USP, Dept Biol Sci LCB, ESALQ, BR-13418900 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Plant Cell Reports; v. 28, n. 8, p. 1169-1177, AUG 2009.
• Citações Web of Science: 15

Resumo

We analyzed the impact of ethylene and auxin disturbances on callus, shoots and Agrobacterium rhizogenes-induced hairy root formation in tomato (Solanum lycopersicum L.). The auxin low-sensitivity dgt mutation showed little hairy root initiation, whereas the ethylene low-sensitivity Nr mutation did not differ from the control Micro-Tom cultivar. Micro-Tom and dgt hairy roots containing auxin sensitivity/biosynthesis rol and aux genes formed prominent callus onto media supplemented with cytokinin. Under the same conditions, Nr hairy roots did not form callus. Double mutants combining Rg1, a mutation conferring elevated shoot formation capacity, with either dgt or Nr produced explants that formed shoots with little callus proliferation. The presence of rol + aux genes in Rg1 hairy roots prevented shoot formation. Taken together, the results suggest that although ethylene does not affect hairy root induction, as auxin does, it may be necessary for auxin-induced callus formation in tomato. Moreover, excess auxin prevents shoot formation in Rg1. (AU)

• Processo FAPESP: 02/00329-8 - Estudo das bases genéticas e bioquímicas da competência para regeneração in vitro utilizando micro tomateiro (Lycopersicon esculentum cv Micro-Tom) como modelo experimental
• Beneficiário: Lázaro Eustaquio Pereira Peres
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 03/03940-2 - Obtenção e uso de plantas de tomateiro (Lycopersicon esculentum cv Micro-Tom) transformados com Agrobacterium rhizogenes para o estudo da competência organogenética in vitro
• Beneficiário: Joni Esrom Lima
• Modalidade de apoio: Bolsas no Brasil - Mestrado