Infection kinetics of human adenovirus serotype 41 in HEK 293 cells

Autor(es)

Siqueira-Silva, Joselma ^[1] ; Yeda, Fernanda Perez ^[1] ; Favier, Anne-Laure ^[2] ; Mezin, Paulette ^[3] ; Silva, Misael Leonardo ^[1] ; Barrella, Karina Medici ^[1] ; Mehnert, Dolores Ursula ^[1] ; Fender, Pascal ^[2] ; Harsi, Charlotte Marianna ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Biol Mol Adenovirus, Dept Microbiol, Inst Ciencias Biomed, BR-05508900 Sao Paulo - Brazil

^[2] Inst Biol Struct Jean Pierre Ebel, Grenoble - France

^[3] CHU Grenoble, La Tronche - France

• Tipo de documento: Artigo Científico
• Fonte: Memórias do Instituto Oswaldo Cruz; v. 104, n. 5, p. 736-744, AUG 2009.
• Citações Web of Science: 10

Resumo

The purpose of this work was to acquire an overview of the infectious cycle of HAdV-41 in permissive HEK 293 cells and compare it to that observed with the prototype of the genus, Human adenovirus C HAdV-2. HEK 293 cells were infected with each virus separately and were harvested every 12 h for seven days. Infection kinetics were analysed using confocal and electronic microscopy. The results show that, when properly cultivated, HAdV-41 was not fastidious. It had a longer multiplication cycle, which resulted in the release of complete viral particles and viral stocks reached high titres. After 60 h of infection, the export of viral proteins from the infected cell to the extracellular milieu was observed, with a pattern similar to that previously described for HAdV-2 penton-base trafficking after 30 h of infection. HAdV-41 had a non-lytic cycle and the infection spread from the first infected cell to its neighbours. The release process of the viral particles is unknown. The results observed for HAdV-41 infection in HEK 293 cells show how different this virus is from the prototype HAdV-2 and provides information for the development of this vector for use in gene therapy. (AU)

• Processo FAPESP: 02/13859-5 - Estudo de interação do adenovirus sorotipo 41 (HADV-41) com a célula hospedeira
• Beneficiário: Joselma Siqueira Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 00/00176-1 - Estudo dos receptores celulares para Adenovírus humano sorotipo 41 (HAdV-41)
• Beneficiário: Charlotte Marianna Hársi
• Modalidade de apoio: Auxílio à Pesquisa - Regular