| Full text | |
| Author(s): |
Siqueira-Silva, Joselma
[1]
;
Yeda, Fernanda Perez
[1]
;
Favier, Anne-Laure
[2]
;
Mezin, Paulette
[3]
;
Silva, Misael Leonardo
[1]
;
Barrella, Karina Medici
[1]
;
Mehnert, Dolores Ursula
[1]
;
Fender, Pascal
[2]
;
Harsi, Charlotte Marianna
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Sao Paulo, Lab Biol Mol Adenovirus, Dept Microbiol, Inst Ciencias Biomed, BR-05508900 Sao Paulo - Brazil
[2] Inst Biol Struct Jean Pierre Ebel, Grenoble - France
[3] CHU Grenoble, La Tronche - France
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Memórias do Instituto Oswaldo Cruz; v. 104, n. 5, p. 736-744, AUG 2009. |
| Web of Science Citations: | 10 |
| Abstract | |
The purpose of this work was to acquire an overview of the infectious cycle of HAdV-41 in permissive HEK 293 cells and compare it to that observed with the prototype of the genus, Human adenovirus C HAdV-2. HEK 293 cells were infected with each virus separately and were harvested every 12 h for seven days. Infection kinetics were analysed using confocal and electronic microscopy. The results show that, when properly cultivated, HAdV-41 was not fastidious. It had a longer multiplication cycle, which resulted in the release of complete viral particles and viral stocks reached high titres. After 60 h of infection, the export of viral proteins from the infected cell to the extracellular milieu was observed, with a pattern similar to that previously described for HAdV-2 penton-base trafficking after 30 h of infection. HAdV-41 had a non-lytic cycle and the infection spread from the first infected cell to its neighbours. The release process of the viral particles is unknown. The results observed for HAdV-41 infection in HEK 293 cells show how different this virus is from the prototype HAdV-2 and provides information for the development of this vector for use in gene therapy. (AU) | |