| Texto completo | |
| Autor(es): |
Alexandre‚ C.S.
;
Volpini‚ R.A.
;
Shimizu‚ M.H.
;
Sanches‚ T.R.
;
Semedo‚ P.
;
di Jura‚ V.L.
;
Camara‚ N.O.
;
Seguro‚ A.C.
;
Andrade‚ L.
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Stem Cells; v. 27, n. 3, p. 682-692, 2009. |
| Resumo | |
Progressive renal failure continues to be a challenge. The use of bone marrow cells represents a means of meeting that challenge. We used lineage-negative (Lin(-)) cells to test the hypothesis that Lin(-) cell treatment decreases renal injury. Syngeneic Fischer 344 rats were divided into four groups: sham ( laparotomy only, untreated); Nx (five-sixth nephrectomy and untreated); NxLC1 (five-sixth nephrectomy and receiving 2 x 10(6) Lin(-) cells on postnephrectomy day 15); and NxLC3 (five-sixth nephrectomy and receiving 2 x 10(6) Lin(-) cells on postnephrectomy days 15, 30, and 45). On postoperative day 16, renal mRNA expression of interleukin (IL)-1 beta, tumor necrosis factor-alpha, and IL-6 was lower in NxLC rats than in Nx rats. On postnephrectomy day 60, NxLC rats presented less proteinuria, glomerulosclerosis, anemia, renal infiltration of immune cells, and protein expression of monocyte chemoattractant protein-1, as well as decreased interstitial area. Immunostaining for proliferating cell nuclear antigen showed that, in comparison with sham rats, Nx rats presented greater cell proliferation, whereas NxLC1 rats and NxLC3 rats presented less cell proliferation than did Nx rats. Protein expression of the cyclin-dependent kinase inhibitor p21 and of vascular endothelial growth factor increased after nephrectomy and decreased after Lin(-) cell treatment. On postnephrectomy day 120, renal function (inulin clearance) was significantly better in Lin(-) cell-treated rats than in untreated rats. Lin(-) cell treatment significantly improved survival. These data suggest that Lin(-) cell treatment protects against chronic renal failure. STEM CELLS 2009; 27: 682-692 (AU) | |
| Processo FAPESP: | 07/07139-3 - Investigando o papel da heme-oxigenase 1 em diferentes processos inflamatórios renais em modelos animais |
| Beneficiário: | Niels Olsen Saraiva Câmara |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 05/56056-8 - O papel das stem cells de medula óssea na prograssao da doença renal em modelo experimental |
| Beneficiário: | Lucia da Conceição Andrade |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 06/56320-0 - Participação do p53 e de seus substratos Bax e p21 na insuficiência renal aguda induzida pela sépsis: influência do tratamento com pifithrin-alfa e N-acetilcisteína |
| Beneficiário: | Rildo Aparecido Volpini |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 06/00620-5 - Análise dos células tronco mesenquimais em modelos de isquemia renal aguda e crônica |
| Beneficiário: | Patricia Semedo Kuriki |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |