Identification of a metallothionein in Synechococcus by capillary electrophoresis hyphenated with inductively coupled plasma mass spectrometry

Autor(es)

LAVORANTE‚ A.F. ; GINÉ‚ M.F. ; GERVASIO‚ A.P.G. ; MIRANDA‚ C.E.S. ; FIORE‚ M.F. ; BELLATO‚ C.M. ; CARRILHO‚ E.

• Tipo de documento: Artigo Científico
• Fonte: Analytical Sciences; v. 19, n. 12, p. 1611-1616, 2003.

Resumo

home-made system hyphenating capillary electrophoresis with an inductively coupled plasma mass spectrometer (CE-ICP-MS) for cadmium speciation of protein-binding and free cadmium ions in solution is presented. The CE-ICP-MS interface consisted of an acrylic block with an internal volume ca. 20 muL in which a platinum electrode, a capillary column, and a connection to an ICP nebulizer were inserted. A make-up electrolyte solution containing 50 mmol L-1 Tris-HCl buffer solution (pH 9.0) was continuously flowed through the interface to the ICP nebulizer. The separation of free Cd ions, Cd-cysteine, and Cd bounded to metallothionein (MT) isoforms from rabbit liver was carried out by capillary electrophoresis, and the analytes were detected by ICP-MS. The feasibility to isolate metallothionein compounds extracted from the cyanobacterium Synechococcus PCC7942 was demonstrated. The Cd binding proteins were induced in Synechococcus PCC7942 and further analyzed by CE ICP-MS. (AU)

• Processo FAPESP: 99/07185-7 - Identificação e análise molecular de genes patogênicos envolvidos na interação citros-Xylella fastidiosa
• Beneficiário: Cláudia de Mattos Bellato
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores
• Processo FAPESP: 98/05011-9 - Especiação de íons inorgânicos, organo-metálicos e complexos metal-ligantes por eletroforese capilar empregando sistemas de análise em fluxo com detecção espectrométrica
• Beneficiário: Maria Fernanda Georgina Gine Rosias
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 01/08790-3 - Expressao do gene da metalotioneina de synechococcus pcc 7942 e analise estrutural da proteina.
• Beneficiário: Marli de Fátima Fiore
• Modalidade de apoio: Auxílio à Pesquisa - Regular