| Texto completo | |
| Autor(es): |
Duarte‚ E.L.
;
Oliveira‚ T.R.
;
Alves‚ D.S.
;
Micol‚ V.
;
Lamy‚ M.T.
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Langmuir; v. 24, n. 8, p. 4041-4049, 2008. |
| Resumo | |
Barbaloin is a bioactive glycosilated 1,8-dihydroxyanthraquinone present in several exudates from plants, Such as Aloe vera, which are used for cosmetic or food purposes. It has been shown that barbaloin interacts with DMPG (dimyristoylphosphatidylglycerol) model membranes, altering the bilayer structure (Alves, D. S.; Perez-Fons, L.; Estepa, A.; Micol, V. Biochem. Pharm. 2004, 68, 549). Considering that ESR (electron spin resonance) of spin labels is one of the best techniques to monitor structural properties at the molecular level, the alterations caused by the anthraquinone barbaloin on phospholipid bilayers will be discussed here via the ESR signal of phospholipid spin probes intercalated into the membranes. In DMPG at high ionic strength (10 mM Hepes pH 7.4 + 100 mM NaCl), a system that presents a gel-fluid transition around 23 degrees C, 20 mol % barbaloin turns the gel phase more rigid, does not alter much the fluid phase packing, but makes the lipid thermal transition less sharp. However, in a low-salt DMPG dispersion (10 mM Hepes pH 7.4 + 2 mM NaCl), which presents a rather complex gel-fluid thermal transition (Lamy-Freund, M. T.; Riske, K. A. Chem. Phys. Lipids 2003, 122, 19), barbaloin strongly affects bilayer structural properties, both in the gel and fluid phases, extending the transition region to much higher temperature values. The position of barbaloin in DMPG bilayers will be discussed on the basis of ESR results, in parallel with data from sample viscosity, DSC (differential scanning calorimetry), and SAXS (small-angle X-ray scattering). (AU) | |
| Processo FAPESP: | 01/11721-3 - Agregados anfifílicos em meio aquoso: propriedades térmicas e estruturais e interações com moléculas de interesse biológico |
| Beneficiário: | Maria Teresa Moura Lamy |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |