| Texto completo | |
| Autor(es): |
Silva Neto‚ J.F.
;
Koide‚ T.
;
Gomes‚ S.L.
;
Marques‚ M.V.
Número total de Autores: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FEMS Microbiology Letters; v. 210, n. 1, p. 105-110, 2002. |
| Resumo | |
As a first approach to generate mutations by DNA insertion, we have developed a shuttle vector, called pSP3. which replicates both in Escherichia coli and in Xylella. Vector pSP3 was constructed by ligating to the E coli plasmid pBluescript, a kanamycin resistance gene under the control of the Xylella 16S rRNA promoter and the indigenous Xylella plasmid pXF1.3. Transformation experiments have shown that pSP3 replicates stably in Xylella. When a DNA fragment encompassing part of the Xylella xpsD gene was cloned into pSP3, specific integration of the construct into this gene was observed in 10% of the transformants, as early as after two passages of the culture, These results indicate that this vector can be used to generate site-specific gene disruption by homologous recombination in Xylella fastidiosa. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies. (AU) | |
| Processo FAPESP: | 98/16251-0 - Establishment of conditions for the disruption genes potentially involved in Xylella fastidiosa pathogenesis |
| Beneficiário: | Suely Lopes Gomes |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa GENOMA |