| Texto completo | |
| Autor(es): |
Boscariol‚ RL
;
Almeida‚ WAB
;
Derbyshire‚ M.
;
Mourao Filho‚ FAA
;
Mendes‚ BMJ
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Plant Cell Reports; v. 22, n. 2, p. 122-128, 2003. |
| Resumo | |
A new method for obtaining transgenic sweet orange plants was developed in which positive selection (Positech) based on the Escherichia coli phosphomannose-isomerase (PMI) gene as the selectable marker gene and mannose as the selective agent was used. Epicotyl segments from in vitro-germinated plants of Valencia, Hamlin, Natal and Pera sweet oranges were inoculated with Agrobacterium tumefaciens EHA101-pNOV2116 and subsequently selected on medium supplemented with different concentrations of mannose or with a combination of mannose and sucrose as a carbon source. Genetic transformation was confirmed by PCR and Southern blot. The transgene expression was evaluated using a chlorophenol red assay and isoenzymes. The transformation efficiency rate ranged from 3% to 23.8%, depending on cultivar. This system provides an efficient manner for selecting transgenic sweet orange plants without using antibiotics or herbicides. (AU) | |
| Processo FAPESP: | 99/04073-3 - Optimization of in vitro regeneration systems and establishment of a genetic transformation protocol for Citrus SP |
| Beneficiário: | Beatriz Madalena Januzzi Mendes |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa GENOMA |