| Texto completo | |
| Autor(es): |
Fuller‚ N.
;
Benatti‚ C.R.
;
Peter Rand‚ R.
Número total de Autores: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOPHYSICAL JOURNAL; v. 85, n. 3, p. 1667-1674, 2003. |
| Resumo | |
Phosphatidylserine (PS), an anionic phospholipid of significant biological relevance, forms a multilamellar phase in water with net negative surface charge at pH 7.0. In this study we mixed dioleoyIPS (DOPS) with reverse hexagonal (H-II)-forming phosphatidylethanolamine (DOPE), and used x-ray diffraction and osmotic stress to quantify its spontaneous curvature (1/R-0p) and bending modulus (K-cp). The mixtures were stable H-II phases from 5 to 30 mol % PS, providing 16 wt % tetradecane (td) was also added to relieve chain-packing stress. The fully hydrated lattice dimension increased with DOPS concentration. Analysis of structural changes gave an apparent R-0p for DOPS of 1144 Angstrom; opposite in sign and relatively. at compared to DOPE (-30 Angstrom). Osmotic stress of the H-II phases did not detect a significantly different bending modulus (K-cp) for DOPS as compared to DOPE. At pH less than or equal to 4.0, DOPS (with no td) adopted the H-II phase on its own, in agreement with previous results, suggesting a reversal in curvature upon protonation of the serine headgroup. In contrast, when td was present, DOPS/td formed a lamellar phase of limited swelling whose dimension increased with pH. DOPS/DOPE/td mixtures formed H-II phases whose dimension increased both with pH and with DOPS content. With tetradecane, estimates put 1/R-0p for DOPS at pH 2.1 at zero. Tetradecane apparently affects the degree of dissociation of DOPS at low pH. (AU) | |
| Processo FAPESP: | 00/03545-8 - Role of cholesterol in lipofection |
| Beneficiário: | Carlos Roberto Benatti |
| Modalidade de apoio: | Bolsas no Exterior - Pesquisa |