Immunohistochemical and molecular analysis of caveolin-1 expression in canine mammary tumors

Autor(es)

Zuccari, D. A. P. C. ^{[1, 2]} ; Castro, R. ^[1] ; Gavioli, A. F. ^[3] ; Mancini, U. M. ^[4] ; Frade, C. S. ^[1] ; Leonel, C. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Fac Med Sao Jose do Rio Preto, Lab Invest Mol Canc, Sao Jose Do Rio Preto, SP - Brazil

^[2] Univ Estadual Paulista, Programa Posgrad Genet, Sao Jose Do Rio Preto, SP - Brazil

^[3] Fac Med Sao Jose do Rio Preto, Virol Lab, Sao Jose Do Rio Preto, SP - Brazil

^[4] Fac Med Sao Jose do Rio Preto, Lab Marcadores Mol & Bioinformat Med, Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Genetics and Molecular Research; v. 11, n. 1, p. 153-165, 2012.
• Citações Web of Science: 4

Resumo

Caveolin-1 (Cav-1) is a structural protein present in invaginations of the cell membrane. In human breast cancer, the cav-1 gene is believed to be a tumor suppressor gene associated with inhibition of tumor metastasis. However, little is known about its expression, regulation and function in canine mammary tumors. Expression levels of cav-1 were investigated using real- time PCR and immunohistochemical detection with an anti-human Cav- 1 antibody. Gene expression stability of different samples was analyzed using the geNorm software. Mammary tumors from 51 female dogs were compared to normal mammary tissue from 10 female dogs. Malignant mammary cells showed a loss of Cav-1 expression by quantitative RTPCR and weak Cav-1 staining by immunohistochemistry compared to normal mammary gland tissue. There was a significant relationship between outcome and immunostaining as well as with tumor size, indicating that caveolin subexpression has a positive predictive value and is related to higher survival and smaller tumor size. Our findings indicate that Cav-1 is a potential prognostic marker for canine mammary tumors. (AU)

• Processo FAPESP: 05/51936-0 - Avaliação imuno-histoquímica e molecular dos genes caveolina-1, interleucina-8, laminina-5 e serpin como marcadores prognósticos e preditivos nas neoplasias mamárias em cadelas
• Beneficiário: Debora Aparecida Pires de Campos Zuccari
• Modalidade de apoio: Auxílio à Pesquisa - Regular