Cytotoxicity of fipronil on mice liver cells

Autor(es)

de Oliveira, Patricia Rosa ^[1] ; Bechara, Gervasio Henrique ^[2] ; Denardi, Sandra Eloisi ^[1] ; Oliveira, Rodrigo Juliano ^[3] ; Camargo Mathias, Maria Izabel ^[1]

Afiliação do(s) autor(es)

^[1] UNESP, Inst Biociencias, Dept Biol, BR-13506900 Rio Claro, SP - Brazil

^[2] UNESP, Dept Patol Vet, FCAV, BR-14884900 Jaboticabal, SP - Brazil

^[3] Univ Estadual Londrina, Dept Biol Geral, Ctr Ciencias Biol, Uel - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROSCOPY RESEARCH AND TECHNIQUE; v. 75, n. 1, p. 28-35, JAN 2012.
• Citações Web of Science: 16

Resumo

In the present study, mice livers were examined following exposure to different doses of fipronil (15, 25, and 50 mg/kg). Histological and histochemical techniques were used to determine the cytotoxic potential of this compound and to assess the damage it caused to livers. Mice were divided into four groups: control group and groups I, II, and III were exposed to 15, 25, and 50 mg/kg fipronil, respectively. Our findings revealed cytological, morphohistological, and histochemical alterations in liver cells of animals from groups I, II, and III compared to group control animals. These changes included Kupffer-cell proliferation, hepatocyte hypertrophy, accumulation and distribution of proteins, polysaccharides, lipids, and vacuoles in the cytoplasm of hepatocytes, and congestion of blood vessels. These phenotypes mainly characterize the following: (a) autophagic processes, (b) steatosis, and (c) cell death by necrosis, which demonstrate the damage caused by fipronil on nontarget organisms in artificial conditions. Microsc. Res. Tech., 2012. (c) 2011 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 06/52599-0 - Avaliação dos efeitos do fipronil (ingrediente ativo do Frontline) em ovários de carrapatos Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) e na medula óssea de roedores
• Beneficiário: Patrícia Rosa de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado