Human Leucocytes Response to Viable, Extended Freeze-Drying or Heat-Killed Mycobacterium bovis bacillus Calmette-Guerin

Autor(es)

Moreira, J. ^[1] ; Aragao-Filho, W. C. ^[1] ; Barillas, S. G. ^[2] ; Barbosa, S. M. ^[3] ; Pedroza, L. A. ^[1] ; Condino-Neto, A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Immunol, Inst Biomed Sci, BR-05508000 Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Dept Pharmacol, Sch Med, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Ctr Invest Pediat, Sch Med, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Scandinavian Journal of Immunology; v. 75, n. 1, p. 96-101, JAN 2012.
• Citações Web of Science: 5

Resumo

We investigated the effects of viable, extended freeze-drying (EFD) or heat-killed (HK) Mycobacterium bovis bacillus CalmetteGuerin (BCG) in respiratory burst activity, gene expression of CYBB and NCF1 encoding components of the human phagocyte nicotinamide adenine dinucleotide (NADPH) oxidase, TLR2 expression, and in IL-10 and TNF-a cytokine production by human peripheral blood mononuclear cells (PBMCs). Viable BCG significantly inhibited TLR2 and CYBB gene expression, as well as superoxide release by human PBMC. All BCG stimuli augmented IL-10 release, but only HK BCG or viable BCG increased TNF-a release by PBMCs. Our studies show that viable BCG can impair the NADPH oxidase system activation and the TLR2 route in human PBMCs. As well, different BCG preparations can distinctly influence cytokine production by human PBMCs. (AU)

• Processo FAPESP: 04/14522-0 - Estudo de diferentes preparacoes do mycobacterium bovis bacilo de calmette-guerin sobre a liberacao de citocinas e expressao genica de componentes do sistema nadph oxidase na linhagem mielomonocitica ...
• Beneficiário: Juliana Moreira
• Modalidade de apoio: Bolsas no Brasil - Doutorado