| Texto completo | |
| Autor(es): |
Goncalves, F. L. L.
[1]
;
de Souza, G. F. P.
[2]
;
Schmidt, A. F.
[3]
;
Regis, A. C.
[1]
;
de Oliveira, M. G.
[2]
;
Sbragia, L.
[3]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Campinas, Dept Surg, Sch Med Sci, Campinas, SP - Brazil
[2] Univ Estadual Campinas, UNICAMP, Inst Chem, Campinas, SP - Brazil
[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Div Pediat Surg, Dept Surg & Anat, BR-14048900 Ribeirao Preto - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | EUROPEAN JOURNAL OF PEDIATRIC SURGERY; v. 21, n. 6, p. 362-365, DEC 2011. |
| Citações Web of Science: | 2 |
| Resumo | |
Intestinal damage due to gastroschisis (G), an anomaly found with increasing incidence by pedriatic surgeons, is intimately associated with endogenous nitric oxide (NO) production and NO synthase (NOS) expression. Aim: Aim of the study was to evaluate NO production and NOS isoforms in the intestine and amniotic fluid (AF) using a rat model of gastroschisis. Methods: A gastroschisis rat model was surgically created at 18.5 days of gestation (term = 22 days). 3 groups of 12 fetuses each were studied: control (C), sham (S) and (G). Morphometric data of body weight (BW), intestinal weight (IW) and the IW/BW ratio were evaluated and compared. Indirect quantification of NO (nitrite and nitrate - NOx) was analyzed by chemiluminescence, and the expression of the 3 isoforms was analyzed by Western blotting. Results: Group G showed an increase in IW and IW/BW compared with groups C and S. IW: G = 0.27 +/- 0.06, C = 0.20 +/- 0.02, S = 0.20 +/- 0.02 (p < 0.01); IW/BW: G = 4.11 +/- 0.57, C = 5.21 +/- 1.04, S = 5.18 +/- 1.23 (p < 0.05). NO in the G group was lower in the intestine and higher in AF, as opposed to C and S, where it had increased in the intestine and decreased in AF. Intestinal NOx: G = 0.85 +/- 0.28, C = 1.86 +/- 0.82, S = 1.80 +/- 0.69 (p < 0.05); NOx in AF: G = 161.87 +/- 52.11, C = 6.99 +/- 5.45, S = 48.73 +/- 13.183 (p < 0.001). Conclusion: The intestinal inflammation in gastroschisis promotes the release of nitric oxide to the environment (AF). Perhaps NO in the AF may be an inflammatory marker for G. (AU) | |
| Processo FAPESP: | 07/55877-3 - Doadores de óxido nítrico para o tratamento da síndrome metabólica: mecanismos de ação e desenvolvimento de formulações |
| Beneficiário: | Gabriela Freitas Pereira de Souza |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 08/51487-9 - Avaliacao da protecao das alcas intestinais fetais utilizando hidrogel (biomaterial) e oxido nitrico no modelo experimental de gastrosquise |
| Beneficiário: | Lourenço Sbragia Neto |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |