Antigenotoxicity of artepillin C in vivo evaluated by the micronucleus and comet assays

Autor(es)

Bentes Monteiro Neto, Moacir de Azevedo ^[1] ; de Souza Lima, Ildercilio Mota ^[1] ; Furtado, Ricardo Andrade ^[2] ; Bastos, Jairo Kenupp ^[2] ; da Silva Filho, Ademar Alves ^[3] ; Tavares, Denise Crispim ^[1]

Afiliação do(s) autor(es)

^[1] Univ Franca, BR-14404600 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Sao Paulo - Brazil

^[3] Univ Fed Juiz Fora, Dept Farmaceut, Fac Farm, BR-36035900 Juiz De Fora, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF APPLIED TOXICOLOGY; v. 31, n. 8, p. 714-719, NOV 2011.
• Citações Web of Science: 8

Resumo

Artepillin C (3,5-diprenyl-p-coumaric acid), a major compound found in Brazilian green propolis and Baccharis dracunculifolia, shows anti-inflammatory, antibacterial, antiviral, antioxidant and antitumoral activities, among others. The aim of this study was to evaluate the genotoxic potential of artepillin C and its ability to prevent the chemically induced chromosome breakage or loss and the primary DNA damage using the micronucleus and comet assays in male Swiss mice, respectively. The animals were treated by gavage with different doses of artepillin C (0.4, 0.8 and 1.6 kg(-1) b.w.). For the antigenotoxicity assays, the different doses of artepillin C were administered simultaneously to doxorubicin (DXR; micronucleus test; 15 mg kg(-1) b.w.) and to methyl methanesulfonate (MMS; comet assay; 40 mg kg(-1) b.w.). The results showed that artepillin C itself was not genotoxic in the mouse micronucleus and comet assays. In the animals treated with artepillin C and DXR, the number of micronucleated reticulocytes was significantly lower in comparison with the animals treated only with DXR. Regarding antigenotoxicity, artepillin C at the tested doses significantly reduced the extent of DNA damage in liver cells induced by MMS. Copyright (C) 2011 John Wiley \& Sons, Ltd. (AU)

• Processo FAPESP: 07/07211-6 - Estudo da atividade mutagênica e/ou antimutagênica da Baccharis dracunculifolia e do Artepelin C em células de mamíferos
• Beneficiário: Denise Crispim Tavares Barbosa
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/05944-1 - Avaliação in vivo do potencial mutagênico/genotóxico e antimutagênico/antigenotóxico do Artepelin C
• Beneficiário: Ildercílio Mota de Souza Lima
• Linha de fomento: Bolsas no Brasil - Iniciação Científica