Effect of growth hormone on in vitro osteogenesis and gene expression of human osteoblastic cells is donor-age-dependent

Autor(es)

Crippa, Grasiele E. ; Beloti, Marcio M. ; Cardoso, Cristina R. ; Silva, João S. ; Rosa, Adalberto L. ^[5]

• Tipo de documento: Artigo Científico
• Fonte: Journal of Cellular Biochemistry; v. 104, n. 2, p. 369-376, May 2008.
• Área do conhecimento: Ciências da Saúde - Odontologia
• Assunto(s): Cirurgia bucomaxilofacial Expressão gênica Hormônio do crescimento Osteogênese Osteoblastos

Resumo

It has been demonstrated that the effect of GH on bone tissue is reduced with aging. In this study we tested the hypothesis that the action of GH on osteoblastic cells is donor-age-dependent by investigating the effect of GH on the development of osteoblastic phenotype in cultures of cells from adolescents (13-16 years old), young adults (18-35 years old), and adults (36-49 years old). Osteoblastic cells derived from human alveolar bone were cultured with or without GH for periods of up to 21 days, and parameters of in vitro osteogenesis and gene expression of osteoblastic markers were evaluated. GH increased culture growth, collagen content and alkaline phosphatase (ALP) activity in cultures from adolescents and young adults, whereas non-significant effect was observed in cultures from adults. While GH significantly increased the bone-like formation in cultures from adolescents, a slightly effect was observed in cultures from young adults and no alteration was detected in cultures from adults. Results from real-time PCR demonstrated that GH upregulated ALP, osteocalcin, type I collagen, and Cbfa1 mRNA levels in cultures from adolescents. In addition, cultures from young adults showed higher ALP mRNA expression and the expression of all evaluated genes was not affected by GH in cultures from adults. These results indicate that the GH effect on both in vitro osteogenesis and gene expression of osteoblastic markers is donor-age-dependent, being more pronounced on cultures from adolescents. (AU)

• Processo FAPESP: 04/13756-7 - Efeito do hormonio de crescimento (gh) sobre a osteogenese em cultura de osteoblastos humanos.
• Beneficiário: Grasiele Edilaine Crippa
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 03/09767-0 - Osteogênese em titânio: avaliação in vitro dos efeitos de diferentes métodos de estimulação
• Beneficiário: Adalberto Luiz Rosa
• Modalidade de apoio: Auxílio à Pesquisa - Temático