2D-DIGE analysis of mango (Mangifera indica L.) fruit reveals major proteomic changes associated with ripening

Autor(es)

Andrade, Jonathan de Magalhaes ^[1] ; Toledo, Tatiana Torres ^[1] ; Nogueira, Silvia Beserra ^[1] ; Cordenunsi, Beatriz Rosana ^{[2, 1]} ; Lajolo, Franco Maria ^{[2, 1]} ; Oliveira do Nascimento, Joao Roberto ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, FCF, Dept Food Sci & Expt Nutr, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, NAPAN, Food & Nutr Res Ctr, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROTEOMICS; v. 75, n. 11, p. 3331-3341, JUN 18 2012.
• Citações Web of Science: 37

Resumo

A comparative proteomic investigation between the pre-climacteric and climacteric mango fruits (cv. Keitt) was performed to identify protein species with variable abundance during ripening. Proteins were phenol-extracted from fruits, cyanine-dye-labeled, and separated on 2D gels at pH 4-7. Total spot count of about 373 proteins spots was detected in each gel and forty-seven were consistently different between pre-climacteric and climacteric fruits and were subjected to LC-MS/MS analysis. Functional classification revealed that protein species involved in carbon fixation and hormone biosynthesis decreased during ripening, whereas those related to catabolism and the stress-response, including oxidative stress and abiotic and pathogen defense factors, accumulated. In relation to fruit quality, protein species putatively involved in color development and pulp softening were also identified. This study on mango proteomics provides an overview of the biological processes that occur during ripening. (C) 2012 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 09/02658-8 - Análise comparativa dos proteomas de mangas nos estágios pré-climatérico e climatérico e identificação de proteínas com variação de expressão
• Beneficiário: Jonathan de Magalhães Andrade
• Modalidade de apoio: Bolsas no Brasil - Mestrado