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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Subgingival microbiota from Cebus apella (capuchin monkey) with different periodontal conditions

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Autor(es):
Gaetti-Jardim, Jr., Elerson [1, 2] ; Monti, Lira Marcela [1] ; Nicolas Ciesielski, Francisco Isaak [1] ; Gaetti-Jardim, Ellen Cristina [1] ; Okamoto, Ana Claudia [1] ; Schweitzer, Christiane Marie [3] ; Avila-Campos, Mario Julio [2]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] UNESP Univ Estadual Paulista, Dept Pathol & Oral Diagnost, Sch Dent, BR-16015050 Aracatuba, SP - Brazil
[2] Univ Sao Paulo, Anaerobe Lab, Dept Microbiol, Inst Biomed Sci, BR-2415242 Sao Paulo - Brazil
[3] UNESP Univ Estadual Paulista, Dept Math, Engn Fac Ilha Solteira, BR-15385000 Ilha Solteira, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo de Revisão
Fonte: Anaerobe; v. 18, n. 3, p. 263-269, JUN 2012.
Citações Web of Science: 12
Resumo

This present study evaluated the subgingival microbiota of the Cebus apella with different periodontal conditions kept by the Tufted Capuchin Monkey Procreation Center (Sao PauloState University - UNESP) or free-ranging monkeys. For this purpose, clinical specimens of subgingival biofilm were collected from 52 monkeys, of both genders, 40 kept in captivity and 12 free-ranging monkeys. The primates were submitted to periodontal evaluation and biofilm samples were transferred to VMGA III transport medium and ultrapure water. The microbiota was cultivated in selective and non-selective culture media and microbial DNA was extracted and the presence of periodontal pathogens was evaluated using PCR and real-time PCR. The actinomycetes, fusobacteria, Campylobacter rectus, Eikenella corrodens, black-pigmented Gram-negative anaerobic rods, Tannerella forsythia, staphylococci and streptococci represent the predominantly detected microorganisms. Aggregatibacter actinomycetemcomitans, Dialister pneumosintes and Prevotella nigrescens were rarely observed, whereas Treponema denticola was not found. Populations of C. rectus, E. corrodens, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia, T forsythia and the total microbial load were significantly higher in animals with bone loss and, in smaller extension, in animals with gingival bleeding. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 07/54851-0 - Avaliação da microbiota bucal de pacientes submetidos à radioterapia para tratamento de lesões malignas de cabeça e pescoço
Beneficiário:Elerson Gaetti Jardim Júnior
Modalidade de apoio: Auxílio à Pesquisa - Regular