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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Subgingival microbiota from Cebus apella (capuchin monkey) with different periodontal conditions

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Author(s):
Gaetti-Jardim, Jr., Elerson [1, 2] ; Monti, Lira Marcela [1] ; Nicolas Ciesielski, Francisco Isaak [1] ; Gaetti-Jardim, Ellen Cristina [1] ; Okamoto, Ana Claudia [1] ; Schweitzer, Christiane Marie [3] ; Avila-Campos, Mario Julio [2]
Total Authors: 7
Affiliation:
[1] UNESP Univ Estadual Paulista, Dept Pathol & Oral Diagnost, Sch Dent, BR-16015050 Aracatuba, SP - Brazil
[2] Univ Sao Paulo, Anaerobe Lab, Dept Microbiol, Inst Biomed Sci, BR-2415242 Sao Paulo - Brazil
[3] UNESP Univ Estadual Paulista, Dept Math, Engn Fac Ilha Solteira, BR-15385000 Ilha Solteira, SP - Brazil
Total Affiliations: 3
Document type: Review article
Source: Anaerobe; v. 18, n. 3, p. 263-269, JUN 2012.
Web of Science Citations: 12
Abstract

This present study evaluated the subgingival microbiota of the Cebus apella with different periodontal conditions kept by the Tufted Capuchin Monkey Procreation Center (Sao PauloState University - UNESP) or free-ranging monkeys. For this purpose, clinical specimens of subgingival biofilm were collected from 52 monkeys, of both genders, 40 kept in captivity and 12 free-ranging monkeys. The primates were submitted to periodontal evaluation and biofilm samples were transferred to VMGA III transport medium and ultrapure water. The microbiota was cultivated in selective and non-selective culture media and microbial DNA was extracted and the presence of periodontal pathogens was evaluated using PCR and real-time PCR. The actinomycetes, fusobacteria, Campylobacter rectus, Eikenella corrodens, black-pigmented Gram-negative anaerobic rods, Tannerella forsythia, staphylococci and streptococci represent the predominantly detected microorganisms. Aggregatibacter actinomycetemcomitans, Dialister pneumosintes and Prevotella nigrescens were rarely observed, whereas Treponema denticola was not found. Populations of C. rectus, E. corrodens, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia, T forsythia and the total microbial load were significantly higher in animals with bone loss and, in smaller extension, in animals with gingival bleeding. (C) 2012 Elsevier Ltd. All rights reserved. (AU)