| Texto completo | |
| Autor(es): |
Janegitz, Bruno C.
[1]
;
Medeiros, Roberta A.
[1]
;
Rocha-Filho, Romeu C.
[1]
;
Fatibello-Filho, Orlando
[1]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Univ Fed Sao Carlos, Dept Quim, BR-13560970 Sao Carlos, SP - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | DIAMOND AND RELATED MATERIALS; v. 25, p. 128-133, MAY 2012. |
| Citações Web of Science: | 34 |
| Resumo | |
Tyrosinase (Tyr)-based biosensors might be interesting devices for fast analytical screening of phenols, especially if gold nanoparticles (AuNPs) are used as mediators of the direct electron transfer (DET) reaction between enzyme and electrode substrate. Therefore, by immobilizing Tyr on AuNPs electrodeposited on a boron-doped diamond (BDD) electrode. The best conditions (applied potential and deposition time) to electrodeposit AuNPs on BDD were selected by factorial design; homogeneously distributed quasi-spherical AuNPs (33 nm average diameter) were obtained by applying -0.4 V vs. Ag/AgCl (3 M KCl) for 40 s. Then, Tyr was immobilized on AuNPs previously modified using cystamine and glutaraldehyde. The occurrence of DET between the electrode surface and the Tyr active site was verified by cyclic voltammetry. yielding the following parameter values: formal redox potential, 115 mV vs. Ag/AgCl (3 M KCl); transfer coefficient, 0.45; heterogeneous electron transfer rate constant, 0.032 s(-1). Using square-wave voltammetry (SWV), a sensitive electrochemical reduction of phenol was attained: concentration range, 0.10-11.0 mu M; detection limit, 0.07 mu M; intra-day and inter-day repeatability RSDs, 3.4% and 4.24%, respectively; reasonably good stability (only 15% response decrease after 30 days). Furthermore, the obtained apparent Michaelis-Menten kinetic constant for phenol (13.0 mu M) is quite good when compared to the ones reported in the literature. Hence, the developed Tyr-AuNPs/BDD biosensor exhibits good sensitivity, stability, and reproducibility for the determination of phenol by SWV. (C) 2012 Elsevier B.V. All rights reserved. (AU) | |
| Processo FAPESP: | 08/09893-0 - Desenvolvimento de biossensores utilizando nanotubos de carbono e nanopartículas |
| Beneficiário: | Bruno Campos Janegitz |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 11/00601-9 - Aplicação de eletrodos de diamante dopado com boro e de nitreto de carbono amorfo para a determinação simultânea de fármacos de diferentes grupos terapêuticos em formulações farmacêuticas e em urina |
| Beneficiário: | Roberta Antigo Medeiros |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |