Fourier transform infrared microspectroscopy as a bacterial source tracking tool to discriminate fecal E. coli strains

Autor(es)

Carlos, Camila ^[1] ; Maretto, Danilo A. ^[2] ; Poppi, Ronei J. ^[2] ; Sato, Maria Ines Z. ^[3] ; Ottoboni, Laura M. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Ctr Biol Mol & Engn Genet, BR-13083875 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Inst Quim, BR-13084971 Campinas, SP - Brazil

^[3] Co Tecnol Saneamento Ambiental, BR-05489900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Microchemical Journal; v. 99, n. 1, p. 15-19, 2011.
• Citações Web of Science: 9

Resumo

The aim of this work was to use the FT-IR microspectroscopy technique, a union of a FT-IR spectrometer with a microscope, to discriminate fecal Escherichia coli strains from cows, chickens and humans and to compare the efficiency of this method with the genomic fingerprinting method, BOX-PCR. The obtained BOX-PCR profiles were able to correctly discriminate 93.75% of the chicken strains, 80% of the cow strains and 65% of the human strains. An efficient PLS-DA model was developed, using orthogonal signal correction and the second derivate of the FT-IR spectra. This model allowed the correct discrimination, according to the animal source, of all the E. coli strains analyzed. The bands in the FT-IR spectra that were responsible for the strains discrimination were in the region between 2816 and 3026 cm(-1) wavenumber, described as fatty acids. It was demonstrated that FT-IR microspectroscopy can be a suitable tool for fecal E. coli discrimination, because it is fast, easy to carry out and presents a flexible discrimination power. (C) 2011 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 07/57025-4 - Identificação de marcadores moleculares hospedeiro-especificos de Escherichia coli de águas superficiais do estado de São Paulo
• Beneficiário: Camila Carlos
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 06/07309-3 - Novas estratégias analíticas para estudos envolvendo biomoléculas
• Beneficiário: Elias Ayres Guidetti Zagatto
• Modalidade de apoio: Auxílio à Pesquisa - Temático