Molecular Epidemiology of Neisseria meningitidis Serogroup B in Brazil

Autor(es)

de Filippis, Ivano ^{[1, 2, 3]} ; de Lemos, Ana Paula S. ^[4] ; Hostetler, Jessica B. ^[5] ; Wollenberg, Kurt ^[6] ; Sacchi, Claudio T. ^[7] ; Harrison, Lee H. ^[8] ; Bash, Margaret C. ^[2] ; Prevots, D. Rebecca ^[3]

Afiliação do(s) autor(es)

^[1] Oswaldo Cruz Fdn FIOCRUZ, Natl Qual Control Inst INCQS, Rio De Janeiro - Brazil

^[2] US FDA, Lab Bacterial Polysaccharides, CBER, Bethesda, MD 20014 - USA

^[3] NIAID, Epidemiol Unit, Lab Clin Infect Dis, Div Intramural Res, NIH, Bethesda, MD 20892 - USA

^[4] IAL, Dept Bacteriol, Sao Paulo - Brazil

^[5] JCVI, Rockville, MD - USA

^[6] NIAID, Off Cyberinfrastruct & Computat Biol OCICB, NIH, Bethesda, MD 20892 - USA

^[7] Inst Adolfo Lutz Registro, Dept Immunol, Sao Paulo - Brazil

^[8] Univ Pittsburgh, Infect Dis Epidemiol Res Unit, Pittsburgh, PA - USA

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 7, n. 3, p. e33016, 2012.
• Citações Web of Science: 23

Resumo

Background: Neisseria meningitidis serogroup B has been predominant in Brazil, but no broadly effective vaccine is available to prevent endemic meningococcal disease. To understand genetic diversity among serogroup B strains in Brazil, we selected a nationally representative sample of clinical disease isolates from 2004, and a temporally representative sample for the state of Sao Paulo (1988-2006) for study (n = 372). Methods: We performed multi-locus sequence typing (MLST) and sequence analysis of five outer membrane protein (OMP) genes, including novel vaccine targets fHbp and nadA. Results: In 2004, strain B:4:P1.15,19 clonal complex ST-32/ET-5 (cc32) predominated throughout Brazil; regional variation in MLST sequence type (ST), fetA, and porB was significant but diversity was limited for nadA and fHbp. Between 1988 and 1996, the Sao Paulo isolates shifted from clonal complex ST-41/44/Lineage 3 (cc41/44) to cc32. OMP variation was associated with but not predicted by cc or ST. Overall, fHbp variant 1/subfamily B was present in 80% of isolates and showed little diversity. The majority of nadA were similar to reference allele 1. Conclusions: A predominant serogroup B lineage has circulated in Brazil for over a decade with significant regional and temporal diversity in ST, fetA, and porB, but not in nadA and fHbp. (AU)

• Processo FAPESP: 07/00462-3 - Diversidade genética e clonal da Neisseria meningitidis B no Brasil
• Beneficiário: Claudio Tavares Sacchi
• Modalidade de apoio: Auxílio à Pesquisa - Regular