Patterns of cell proliferation and apoptosis by topographic region in normal Bos taurus vs. Bos indicus crossbreeds bovine placentae during pregnancy

Autor(es)

Facciotti, Patricia R. ^[1] ; Rici, Rose E. G. ^[1] ; Maria, Durvanei A. ^[2] ; Bertolini, Marcelo ^[3] ; Ambrosio, Carlos E. ^[4] ; Miglino, Maria A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Surg, Fac Vet Med & Anim Sci, BR-05508270 Sao Paulo - Brazil

^[2] Butantan Inst, Biochem & Biophys Lab, BR-05503900 Sao Paulo - Brazil

^[3] UDESC Santa Catarina State Univ, Sch Vet Med, BR-88520000 Lages, SC - Brazil

^[4] Univ Sao Paulo, Dept Basic Sci, FZEA, Fac Vet Med, BR-13635900 Pirassununga, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Reproductive Biology and Endocrinology; v. 7, p. 1-7, 2009.
• Citações Web of Science: 8

Resumo

Background: Placental and fetal growth requires high rates of cellular turnover and differentiation, which contributes to conceptus development. The trophoblast has unique properties and a wide range of metabolic, endocrine and angiogenic functions, but the proliferative profile of the bovine placenta characterized by flow cytometry analysis and its role in fetal development are currently uncharacterized. Complete understanding of placental apoptotic and proliferative rates may be relevant to development, especially if related to the pathogenesis of pregnancy losses and placental abnormalities. Methods: In this study, the proliferation activity and apoptosis in different regions of normal bovine placenta (central and boundary regions of placentomes, placentomal fusion, microplacentomes, and interplacentomal regions), from distinct gestation periods (Days 70 to 290 of pregnancy), were analyzed by flow cytometry. Results: Our results indicated that microplacentomes presented a lower number of apoptotic cells throughout pregnancy, with a higher proliferative activity by the end of gestation, suggesting that such structures do not contribute significantly to normal of placental functions and conceptus development during pregnancy. The placentome edges revealed a higher number of apoptotic cells from Day 170 on, which suggests that placentome detachment may well initiate in this region. Conclusion: Variations involving proliferation and apoptotic rates may influence placental maturation and detachment, compromising placental functions and leading to fetal stress, abnormalities in development and abortion, as frequently seen in bovine pregnancies from in vitro fertilization and cloning procedures. Our findings describing the pattern of cell proliferation and apoptosis in normal bovine pregnancies may be useful for unraveling some of the developmental deviations seen in nature and after in vitro embryo manipulations. (AU)

• Processo FAPESP: 07/52137-9 - Influencias da idade gestacional sobre as taxas de proliferacao e apoptose celular em diferentes regioes da placenta de bovinos nao manipulados.
• Beneficiário: Patricia Reginato Facciotti
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 07/59744-8 - Organogenese embrionaria e fetal e interacao da receptora nos animais clonados
• Beneficiário: Maria Angelica Miglino
• Modalidade de apoio: Auxílio à Pesquisa - Regular