Influence of 2% chlorhexidine gel on calcium hydroxide ionic dissociation and its ability of reducing endotoxin

Autor(es)

Correa Signoretti, Fernanda Graziela ; Figueiredo de Almeida Gomes, Brenda Paula ; Montagner, Francisco ^[1] ; Tosello, Fernanda Barrichello ; Jacinto, Rogerio Castilho ^{[2, 3]}

Afiliação do(s) autor(es)

^[1] Univ Fed Rio Grande do Sul, Endodont Area, Dept Conservat Dent, Porto Alegre, RS - Brazil

^[2] Univ Estadual Campinas, Endodont Div, Dept Restorat Dent, Piracicaba Dent Sch, Piracicaba - Brazil

^[3] Univ Fed Pelotas, Endodont Area, Pelotas Dent Sch, Pelotas, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology; v. 111, n. 5, p. 653-658, 2011.
• Citações Web of Science: 13

Resumo

Objective. The aim of this study was to evaluate the influence of 2% chlorhexidine gel (CHX) on pH, calcium release, and Ca(OH)(2) capability of reducing endotoxin. Study design. Calcium release was verified by atomic-absorbance spectrophotometry, and pH was measured with a pHmeter. For endotoxin quantification, extracted human teeth previously contaminated with standard endotoxin were filled with: group I, Ca(OH)(2) + saline solution; group II, Ca(OH)(2) + CHX; and group III, CHX for 14 days. The remaining endotoxin was quantified by using chromogenic quantitative test. Statistical analysis was performed with analysis of variance and Tukey post hoc test (P < .05). Results. Group II released more calcium than group I (P < .05) after 15 days. Groups I and II showed alkaline pH in all periods, and group I showed higher pH values than group II (P < .05) after 30 days. Groups II and III showed significantly more decreased endotoxin than group I. Conclusions. CHX did not interfere with the chemical properties of Ca(OH)(2), in fact even improving its properties of reducing the endotoxin content in root canals in vitro. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011;111: 653-658) (AU)

• Processo FAPESP: 08/58299-3 - Estudo microbiológico e de endotoxinas em canais radiculares de dentes com necrose pulpar e lesão periapical e avaliação da atividade endotóxica de LPS isolados de canais radiculares infectados
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/60500-3 - Investigação da microbiota de canais radiculares infectados de dentes sintomáticos e assintomáticos por cultura e sequenciamento do gene 16S rRNA
• Beneficiário: Rogerio de Castilho Jacinto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 07/58518-4 - Análise da microbiota de canais radiculares de dentes humanos infectados através da cultura, clonagem e sequenciamento do gene 16S rRNA
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular