Genotoxic and mutagenic effects of fipronil on mice

Autor(es)

de Oliveira, Patricia Rosa ^[1] ; Bechara, Gervasio Elenrique ^[2] ; Denardi, Sandra Eloisi ^[1] ; Oliveira, Rodrigo Juliano ^{[1, 3]} ; Camargo Mathias, Maria Izabel ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Biol, Inst Biosci, BR-13506900 Rio Claro, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Anim Pathol, Fac Agron & Vet Sci, BR-14884900 Jaboticabal, SP - Brazil

^[3] Univ Estadual Londrina UEL, Dept Biol Geral, Ctr Ciencias Biol, Londrina, PR - Brazil

• Tipo de documento: Artigo Científico
• Fonte: EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY; v. 64, n. 6, p. 569-573, SEP 2012.
• Citações Web of Science: 18

Resumo

The toxic effects of several compounds on ecosystems are not restricted to ecological disturbances, and may also affect long-term human health. Fipronil is highly efficient in the control of pests, including those resistant to pyrethroid, organophosphate, and carbamate insecticides. Relatively little is known about the action of fipronil in vertebrates. This study was aimed to evaluate the genotoxic and mutagenic potential of this compound in mice exposed to different doses and demonstrates the damage caused by fipronil on non-target organisms in artificial conditions. Mice were divided into five groups: group I = 30% of DL50 (15 mg/kg), group II = 50% of the DL50 (25 mg/kg), group III = DL50 (50 mg/kg), group IV = negative control, and group V = positive control. Peripheral blood was collected for the comet assay (24 h after exposure) and the micronucleus test (24,48 and 72 h after exposure). Our findings revealed that doses of 15 mg/kg (group I) and 25 mg/kg (group II) of fipronil did not have genotoxic or mutagenic effects. Only the highest dose tested (50 mg/kg) induced DNA damage 24 h after exposure, indicating the mutagenic potential of fipronil. Therefore, this or higher doses are not recommended, as they may be toxic to non-target organisms. (C) 2010 Elsevier GmbH. All rights reserved. (AU)

• Processo FAPESP: 06/52599-0 - Avaliação dos efeitos do fipronil (ingrediente ativo do Frontline) em ovários de carrapatos Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) e na medula óssea de roedores
• Beneficiário: Patrícia Rosa de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado