In vitro evaluation of combined hyperthermia and photodynamic effects using magnetoliposomes loaded with cucurbit[7]uril zinc phthalocyanine complex on melanoma

Autor(es)

Bolfarini, Gisele C. ^[1] ; Siqueira-Moura, Marigilson P. ^[1] ; Demets, Gregoire J. F. ^[2] ; Morais, Paulo C. ^[3] ; Tedesco, Antonio C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Lab Fotobiol & Fotomed, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Lab Mat & Interfaces Mol, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Univ Brasilia, Inst Fis, BR-70910900 Brasilia, DF - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 115, p. 1-4, OCT 3 2012.
• Citações Web of Science: 48

Resumo

The aims of this study were two fold; to develop magnetoliposomes (MLs) loaded with zinc phthalocyanine (ZnPc) complexed with cucurbituril (CB) (CB:ZnPc-MLs) and to evaluate their in vitro photodynamic (PD) and/or hyperthermia (HT) effects while using melanoma cells (B16-F10) as model. The liposomal formulations were characterized by both average diameter and zeta potential. The vesicle average size ranged from 150 to 200 nm and the polydispersity index (PdI) from 0.093 to 0.230. The zeta potential was significantly positive with values between 48 and 57 mV. The cell viability (CV) after PD and HT treatments was assessed by colorimetric MTI method. Melanoma cells were initially treated with the liposome formulation without light and magnetic field application, revealing cell viability not different from the control cells (p > 0.05). Photodynamic and hyperthermia assays were also applied separately, demonstrating that PD is more effective than HT in reducing the CV of the neoplastic cells. Combined application of both PD and HT treatments was even more effective in reducing the CV of B16-F10 cells. At the highest light dose (2 J/cm(2)) and under magnetic field activation the CV was about half than PD applied alone. Therefore, the use of the photosensitizer-loaded magnetoliposome for combined photodynamic therapy (PDT) and magnetohyperthermia (MHT) application can be considered as a potential tool to treat malignant melanoma. (C) 2012 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 07/58809-9 - Nanocarreadores contendo ftalocianina de cloroaluminio: desenvolvimento, caracterizacao fisico-quimica e avaliacao in vitro da fotocitotoxicidade em melanoma
• Beneficiário: Marigilson Pontes de Siqueira Moura
• Modalidade de apoio: Bolsas no Brasil - Doutorado