Characterization of a Spodoptera frugiperda multiple nucleopolyhedrovirus isolate that does not liquefy the integument of infected larvae

Autor(es)

Vieira, Corina M. ^[1] ; Tuelher, Edmar S. ^[2] ; Valicente, Fernando H. ^[2] ; Caldas Wolff, Jose Luiz ^[3]

Afiliação do(s) autor(es)

^[1] Univ Mogi das Cruzes, Nucleo Integrado Biotecnol, Mogi Das Cruzes, SP - Brazil

^[2] Ctr Nacl Pesquisa Milho & Sorgo Embrapa, Sete Lagoas, MG - Brazil

^[3] Univ Presbiteriana Mackenzie, CCBS, BR-01302907 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Invertebrate Pathology; v. 111, n. 2, p. 189-192, OCT 2012.
• Citações Web of Science: 9

Resumo

Here we report the characterization of a Spodoptera frugiperda multiple nucleopolyhedrovirus isolate, named SfMNPV-6nd, that does not cause the liquefaction of the host integument. The sequencing of the chitinase A (v-chiA) gene from SfMNPV-6nd revealed that it had a frameshift mutation that greatly reduced size of the putative enzyme. In order to evaluate the suitability of SfMPNV-6nd as a biopesticide, this isolate was compared with the highly virulent SfMNPV-19. Our results showed that the LC50 of the two isolates were not significantly different, but that SfMNPV-6nd took a longer period of time to kill second instar S. frugiperda than SfMNPV-19. (C) 2012 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 07/50209-2 - Clonagem e caracterização molecular de isolados do baculovírus SfMNPV que não causam a degradação de larva infectada
• Beneficiário: José Luiz Caldas Wolff
• Modalidade de apoio: Auxílio à Pesquisa - Regular