Effect of acid etching time on demineralization of primary and permanent coronal dentin

Autor(es)

Salles Scheffel, Debora Lopes ^[1] ; Andalo Tenuta, Livia Maria ^[2] ; Cury, Jaime Aparecido ^[2] ; Hebling, Josimeri ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AMERICAN JOURNAL OF DENTISTRY; v. 25, n. 4, p. 235-238, AUG 2012.
• Citações Web of Science: 5

Resumo

Purpose: To determine the effect of acid etching time on dentin calcium solubilization and to compare the solubilization rate of primary and permanent dentin when etched for the same period of time. Methods: Flat coronal dentin surfaces were produced on primary (n=8) and permanent (n=8) sound teeth. Three 2 mm-diameter areas were delimited on each surface and assigned to 5, 10 or 15 seconds of acid etching. Five microliters of 35% H3PO4 were applied on each area during the preset times, and 4 mu L, were retrieved for analysis. The amount of calcium was determined colorimetrically using Arsenazo III reagent and expressed as mu g Ca/mm(2) of dentin. Data were subjected to two-way ANOVA, Tukey's test and linear regression (alpha=5%). Results: For both primary and permanent dentin, a statistically significant correlation was observed between acid etching time and calcium concentration (primary dentin R-2= 0.79; permanent dentin R-2= 0.73). Mean (sd) values of dissolved primary dentin after 5, 10 and 15 seconds were 1.00 (0.25), 1.74 (0.11) and 2.30 (0.42), whereas for permanent dentin the values were 0.47 (0.13), 0.89 (0.36) and 1.38 (0.20) mu g Ca/mm(2), respectively. Greater calcium solubilization (P< 0.05) was detected for primary dentin than for permanent dentin after all acid etching times. (Am J Dent 2012;25:235-238). (AU)

• Processo FAPESP: 09/07924-8 - Tempo de condicionamento da dentina hígida e afetada por cárie, de dentes decíduos e permanentes: efeito sobre a solubilidade do substrato e produção da união resina-dentina
• Beneficiário: Josimeri Hebling Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/02278-8 - Tempo de condicionamento da dentina hígida e afetada por cárie de dentes decíduos e permanentes: efeito na dissolução do substrato e resistência da união resina-dentina
• Beneficiário: Débora Lopes Salles Scheffel
• Modalidade de apoio: Bolsas no Brasil - Mestrado